产品说明
Fireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.Biotium’sSteady-Luc™HTSassaysystem isahomogeneousfireflyluciferasereportergeneassaykitwithahalf-lifeofabout3hoursforthequantificationoffireflyluciferaseexpressioninmammaliancells,allowingbatchprocessinginmicroplates.Fireflyluciferaseiswidelyusedasareporterforstudyinggeneregulationandfunction,andforpharmaceuticalscreening.Itisaverysensitivegeneticreporterduetothelackofendogenousactivityinmammaliancellsortissues.However,thelightproductionresultingfromthereactionleadstoformationofsuicidaladenyl-oxyluciferinattheenzymesurface.Itresultsinveryshorthalf-lifeofthelightemissionwithaflash-typekinetics.Becauseofthis,flash-typeluminescencemustbedetectedimmediatelyafterinitiatingthereaction,whichisnotcompatIBLewithbatchanalysisofsamples.Biotium’sSteady-Luc™HTSassaysystemisaproprietarymixtureofsubstancesthatmodifytheenzymaticreactiontoproducealonglastingsignal(steadyglow)bypreventingtheformationofadenyl-oxyluciferinattheenzymesurface.Itisahomogeneoushighsensitivityfireflyluciferasereportergeneassaykitwithahalf-lifeofabout3hoursforthequantificationoffireflyluciferaseexpressioninmammaliancells,allowingbatchprocessinginmicroplates.Glow-typeluciferaseassayslikeSteady-Luc™havelowerluminescencesignalcomparedtoflash-typeassays.Thesensitivityandlimitofdetectionoftheassaywilldependonluciferaseexpressionlevelsinyourexperimentalsystemaswellasluminometersensitivity.BiotiumalsooffersSteady-Luc™FireflyHTSAssayKit(Lyophilized)(catalogno.30028L),anewpackagingformatthatincludeslyophilizedassaybufferforconvenientroomtemperatureshippingandstorageat-20°C.KitComponents:D-LuciferinSteady-LucFireflyAssayBufferFeatures:Simple:singlestephomogeneousassay.Glowkinetics:luminescencehalflifeofabout3hours.Economic:saveupto45%comparedtootherbrands.MoreLuciferaseKitsCatalognumberProductDescription30085FireflyLuciferaseAssayKit2.0Flash-typefireflyluciferaseassaykit.30075FireflyLuciferaseAssayKit(Lyophilized)Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.999235XFireflyLysisBufferExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.30028Steady-LucFireflyHTSAssayKitGlow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates.30028LSteady-LucFireflyHTSAssayKit(Lyophilized)Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C.30082RenillaLuciferaseAssayKit2.0Flash-typeRenillaluciferaseassaykit.999125XPassiveLysisBufferExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.30081FireflyandRenillaLuciferaseSingleTubeAssayKitAssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube.99821-100mL1XPassiveLysisBuffer2.0ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well.30020ATP-GloBioluminometricCellViABIlityAssayKitFlash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration.22003MiniCellScrapersMini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates.Referencesn/a
★EB(溴化乙锭):溴化乙锭是一种高度灵敏的荧光染色剂,用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号,可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。
★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm。
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