首页 > Biotium > Biotium/Mix-n-Stain Fluorescent Protein & Tandem Dye Antibody Labeling Kits/92298/25-50-ug-labeling

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Biotium/Mix-n-Stain Fluorescent Protein & Tandem Dye Antibody Labeling Kits/92298/25-50-ug-labeling

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Mix-n-StainFluorescentProtein&TandemDyeAntibodyLabelingKitsutilizearevolutionaryantibodylabelingtechnologythatallowsyoutoeasilylabelyourantibodywithAPC,R-PE,PerCPorAPC-CF750Tinabout4hours.ThelabelingproceduretoleratesmanycommonbuffercomponentsandantibodystABIlizers(seetheproductprotocolunderdownloadsfordetails).Mix-n-StainFluorescentProtein&TandemDyeAntibodyLabelingKitsutilizearevolutionaryantibodylabelingtechnologythatallowsyoutoeasilylabelyourantibodywithAPC,R-PE,PerCPorAPC-CF750Tinabout4hours.Thelabelingproceduretoleratesmanycommonbuffercomponentsandantibodystabilizers(seetheproductprotocolunderdownloadsfordetails).Choosefromkitsizestolabel25-50ug,or50-100ugofyourantibody.Formoreinformation,pleasedownloadtheproductinformationsheetforthisproduct.APCandR-PEbelongtothefamilyofphycobiliproteins,whicharederivedfromcyanobacteriaandeukaryoticalgae.APChasabsorptionmaximaat650andemissionat660nm.Becauseofthehighfluorescencequantumyield(~0.68),APCisbyfarthebrightestdyeforthe650nmwavelengthregion.R-PEhasabsorptionmaximaat496,546,and565nmrespectivelyandemissionat578nm.Becauseofthehighextinctioncoefficient(~1,960,000)andhighfluorescencequantumyield(~0.82),R-PEisbyfarthebrightestdyeforthe565nmwavelengthregion.PerCPisasmall35kDAphycobiliproteinderivedfromredalgae. PerCPhasabsorptionmaximaat482nmandemissionat677nm.TheMix-n-StainAPC-CF750TAntibodyLabelingKitisforlabelinganantibodywithtandemAPC-CF750T(Ex/Em650/780nm)formulti-colorflowcytometry.ItisdesignedfordetectionintheAPC-Cy®7channel.ProductInformationProductdescriptionExcitation/emissionCatalognumberReactionsizeProductprotocolMix-n-Stain™R-PEAntibodyLabelingKit496,546,565/578nm9229825-50ugantibodyPI-Mix-n-StainR-PE9229950-100ugantibodyMix-n-Stain™APCAntibodyLabelingKit650/660nm9230625-50ugantibodyPI-Mix-n-StainAPC9230750-100ugantibodyMix-n-Stain™PerCPAntibodyLabelingKit482/677nm9230825-50ugantibodyPI-Mix-n-StainPerCP9230950-100ugantibodyMix-n-Stain™APC-CF™750AntibodyLabelingKit650/780nm(APC-Cy®7channel)9231025-50ugantibodyPI-Mix-n-StainAPC-CF7509231150-100ugantibodyReferencesn/a

★EB（溴化乙锭）：溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。

★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下，SYBR Green I发出微弱的荧光，但一旦与双链DNA结合后，荧光大大增强。因此，SYBR Green I的荧光信号强度与双链DNA的数量相关，可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm，发射波长最大约为520nm。