产品说明
Mix-n-Stain Fluorescent Protein & Tandem Dye Antibody Labeling Kits utilize a revolutionary antibody labeling technology that allows you to easily label your antibody with APC, R-PE, PerCP or APC-CF750T in about 4 hours. The labeling procedure tolerates many common buffer components and antibody stabilizers (see the product protocol under downloads for details).
Mix-n-Stain Fluorescent Protein & Tandem Dye Antibody Labeling Kits utilize a revolutionary antibody labeling technology that allows you to easily label your antibody with APC, R-PE, PerCP or APC-CF750T in about 4 hours. The labeling procedure tolerates many common buffer components and antibody stabilizers (see the product protocol under downloads for details). Choose from kit sizes to label 25-50 ug, or 50-100 ug of your antibody. For more information, please download the product information sheet for this product.
APC and R-PE belong to the family of phycobiliproteins, which are derived from cyanobacteria and eukaryotic algae.
APC has absorption maxima at 650 and emission at 660 nm. Because of the high fluorescence quantum yield (~0.68), APC is by far the brightest dye for the 650 nm wavelength region.
R-PE has absorption maxima at 496, 546, and 565 nm respectively and emission at 578 nm. Because of the high extinction coefficient (~1,960,000) and high fluorescence quantum yield (~0.82), R-PE is by far the brightest dye for the 565 nm wavelength region.
PerCP is a small 35 kDA phycobiliprotein derived from red algae. PerCP has absorption maxima at 482 nm and emission at 677 nm.
The Mix-n-Stain APC-CF750T Antibody Labeling Kit is for labeling an antibody with tandem APC-CF750T (Ex/Em 650/780 nm) for multi-color flow cytometry. It is designed for detection in the APC-Cy®7 channel.
Product Information
Product descriptionExcitation/emissionCatalog numberReaction size Product protocol
Mix-n-Stain™ R-PE Antibody Labeling Kit496, 546, 565/
578 nm9229825-50 ug antibody
PI-Mix-n-Stain R-PE
9229950-100 ug antibody
Mix-n-Stain™ APC Antibody Labeling Kit650/660 nm9230625-50 ug antibody
PI-Mix-n-Stain APC
9230750-100 ug antibody
Mix-n-Stain™ PerCP Antibody Labeling Kit482/677 nm9230825-50 ug antibody
PI-Mix-n-Stain PerCP
9230950-100 ug antibody
Mix-n-Stain™ APC-CF™750 Antibody Labeling Kit650/780 nm (APC-Cy®7 channel)9231025-50 ug antibody
PI-Mix-n-Stain APC-CF750
9231150-100 ug antibody
References
n/a
★EB(溴化乙锭):溴化乙锭是一种高度灵敏的荧光染色剂,用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号,可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。
★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm。
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