产品说明
CoverGrip™CoverslipSealantisthefirstproductspecificallydesignedforsealingtheedgesofwet-mountedcoverslipsforfluorescencemicroscopy.Unlikenailpolish,CoverGrip™containsnoingredientsthatcanleachintoaqueousmountingmediumandaffectspecimenfluorescence.CoverGrip™CoverslipSealantisthefirstproductspecificallydesignedforsealingtheedgesofwet-mountedcoverslipsforfluorescencemicroscopy.Unlikenailpolish,CoverGrip™ containsnoingredientsthatcanleachintoaqueousmountingmediumandaffectspecimenfluorescence.CoverGrip™ismadewithd-limonene,anatural,environmentally-friendlysolventwithapleasantcitrusaroma,anddriestoformaclear,hard,durablesealalongtheedgesofthecoverslip.Availablein15mLbrush-capapplicatorbottles(23005)orin100mLdropper-caprefillbottles(23005-1).References1.CurrentProtocolsinMouseBIOLOGy(2012).DOI:10.1002/9780470942390.mo1102292.TheJournalofNeuroscience(2015),doi:10.1523/JNEUROSCI.4287-14.20153.CellReports(2015),http://dx.doi.org/10.1016/j.celrep.2015.01.0604.ACSBiomater.Sci.Eng.(2015),doi:10.1021/acSBIomaterials.5b003195.MethodsandProtocols(2015),doi:10.1007/978-1-4939-3124-8_46.AlgalResearch(2016),doi:10.1016/j.algal.2016.03.0167.JExpBiol.(2016),doi:10.1242/jeb.1320848.ScientificReports(2016),doi:10.1038/srep266619.PLoSGenet.(2017),doi:10.1371/journal.pgen.1006603
★EB(溴化乙锭):溴化乙锭是一种高度灵敏的荧光染色剂,用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号,可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。
★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm。
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