DescriptionAdditionalDescription DetailsDescription:Mouse monoclonal antibody to human Transforming Growth Factor beta (TGF-β)Purification: Protein G affinity purifiedProduct Type:Primary antibodyTarget Protein:Human TGF-βImmunogen:TGF-βfrom human plateletsFusion Myeloma: Sp2/0-Ag14Specificity:Western blotting demonstrated that this antibody reacts with the dimeric (25 kDa) and monomeric (12.5 kDa.) forms of TGF-β under both non-reducing and reducing conditions respectively. This antibody recognizes both human platelet-derived and recombinant TGF-β in ELISA.Species Reactivity: Human, others not tested.Host / Isotype: Mouse, IgG1 KappaFormulation: Lyophilized in 0.01M PBS, pH 7.0.Reconstitution:Double distilled water is recommended and to adjust the final concentration to 1.00mg/mL.Storage: Store at -20oCResearch Area:Growth Factors and Their Receptors, AngiogenesisBackground: Transforming growth factor beta (TGF-β) has three isoforms (TGF-β1, TGF-β2, and TGF-β3) with similar functions.The cytokine is a homodimer linked by disulfide bind. Inside cells, the cytokine forms a small latent complex with latent associated peptide (LAP). This small complex binds to latent TGF-β binding protein (LTBP) to be secreted to extra-cellular matrix. Disassociation of the latent proteins from TGF-β results in the release of the cytokine to its receptor. The process is called activation, which can be influenced by various factors, including proteases, metalloproteases, extreme pH, mild acidic condition, reactive oxygen species and integrins.TGF-β is an anti-proliferation factor in normal cells. It increases the synthesis of p15 and p21, which can block the cyclin: CDK complex, and causes cells to stop at G1 phase. The cytokine can induce apoptosis through both SMAD and DAXX pathways. In cancer cells, TGF-β signaling is altered and TGF-β no longer stops cell proliferation.Applications:ELISA: The antibody reacts with TGF-β.Western Blot: This antibody when used at concentration of 5-20ng/mL will allow visualization of 100ng/lane of TGF-β.References:If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude. Additional Additional InformationProduct SpecificitymAb anti-Human TGF-β, 2C5ApplicationEIA, WBSize0.1 mg