产品说明
DescriptionUeyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome .Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.Catalog NumberIW-MA1106Quantity9 mlHostMouseClone1A4IsotypeMouse IgG2aImmunogenN-terminal synthetic decapeptide of α-smooth muscle actin.PurityPurified by the goat anti-mouse IgG affinity chromatography.ConjugateUnconjugatedSpecies ReactivityHuman, mouse, rat. Not tested in other species.Positive ControlSmooth muscle, colon, skin, tonsil.Cellular LocalizationMembrane/cytoplasmicFormReady to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.StorageStore at 2-8 °C. Do not freeze.ApplicationsIHC-P: Pretreatment is NOT required on formalin fixed paraffin sections.IHC-Fr: Not tested.ICC: Not tested.LimitationsThis product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.PrecautionsUsers should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.References1. Kumar, M. S.; Hendrix, J. A.; Johnson, A. D.; Owens, G. K. : Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins. Circ. Res. 92: 840-847, 2003. 2. Ueyama, H.; Bruns, G.; Kanda, N. : Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome 10. Jpn. J. Hum. Genet. 35: 145-150, 1990.
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