产品说明
Descriptionalpha-tubulin (b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.Catalog NumberIW-MA1107Quantity9 mlHostMouseCloneTub-1IsotypeMouse IgG1ImmunogenMicrotubules from chicken embryo brain.PurityPurified by the goat anti-mouse IgG affinity chromatography.ConjugateUnconjugatedSpecies ReactivityHuman, rat. Not tested in other species.Positive ControlBrainCellular LocalizationCytoplasmicFormReady to use solution in PBS with stabilizer and 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.StorageStore at 2-8 °C. Do not freeze.ApplicationsIHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.IHC-Fr: Not tested.ICC: Not tested.LimitationsThis product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.PrecautionsUsers should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.References1. Cowan, N. J.; Dobner, P. R.; Fuchs, E. V.; Cleveland, D. W. : Expression of human alpha-tubulin genes: interspecies conservation of 3-prime untranslated regions. Molec. Cell. Biol. 3: 1738-1745, 1983. 2. Hall, J. L.; Cowan, N. J. : Structural features and restricted expression of a human alpha-tubulin gene. Nucleic Acids Res. 13: 207-223, 1985.
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