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Formalin-fixed paraffin-embedded (FFPE) specimens have been largely ignored because of the recognized difficulty in isolation of proteins from such specimens. The analysis of formalin-fixed paraffin-embedded (FFPE) specimens is desirable because FFPE are the largest and longest time-spanning collections of patient materials in pathology archives around the world. Moreover, because the clinical records are available, and the clinical behavior and outcomes for many of the cases are known, FFPE specimens are the most ideal specimen for translational studies of cancer.The ToPI-F2 is developed specifically to permit the isolation of total proteins from Formalin Fixed Paraffin Embedded (FFPE) specimen for 1D-PAGE and mass spectrometry (Figure 1). Each kit contains all the reagents needed in a ready-to-use format and the standard operating procedure. ToPI-F2 has been used to isolate proteins from FFPE samples stored for up to 5-10 years.The protocol and reagents provided in the kit is sufficient for isolating protein from 10 x 5 (10u thick) specimens. Approximately 5,000 ug/ml – 10,000 ug/ml of total protein can be extracted from 5 sections (~10u thick each) obtained from samples fixed with 10% buffered formalin and stored for up to 5 years. Note that the 1D-gel profile will vary according to the type of tissue, length of storage and fixation protocol. Also, depending on the desired protein yield, type of tissue, formalin fixation protocol and duration of storage, it may be necessary to adjust the number of sections used and protocol to compensate for the inherent difference in tissue types and age of FFPE specimen.Fig 1: 1D-PAGE gel image of ToPI-F2 Human Lung Tissue1D-PAGE gel image of protein isolated from formalin-fixed paraffin embedded (FFPE) human lung tissue using the ToPI-F2 kit. MW is molecular weight marker and lanes 1-4 are proteins isolated from four different lung specimens. Approximately 8ug of total protein were loaded per lane. Order NowEach kit contains optimized and ready to use reagents including:Solution 1Solution 2Solution 3Microfuge tubesValidated protocolK-0017-10 (10 Assays) – $82.00 Click Here to Order on Amazon
isohelix的GeneFiX™唾液DNA微生物组收集装置
微生物研究表明,包括唾液,菌斑,皮肤和肠道在内的许多人类系统中菌群的平衡是健康的关键指标。
微生物组样品的传统收集和存储方法通常包括冷冻,这在冷链运输和存储中总是带来众所周知的后勤挑战,或者通常会使收集过程中的技术可行性变得复杂。
Isohelix已通过新型GeneFiX™唾液微生物组DNA收集设备解决了这些问题。
该设备具有专利的*¹防漏斗,无害缓冲液和简单的操作,非常适合远程或家庭测试。
坚固的样品管经过全面运输测试,达到95KPa,可以邮寄和存放。微生物DNA可以在室温下完全保存在预先填充的缓冲液中至少1年。稳定是即时的,缓冲液可防止细菌生长,因此样品在采样时可准确反映口腔微生物组。该设备和缓冲液不含任何痕量的人类或细菌DNA,因此研究人员可以对其结果的准确性充满信心。
样品管与许多高通量样品处理系统兼容,并包括独特的一维条码和人类可读代码,可实现精确的样品跟踪。
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