The ToPI-DIGE™ Kit contains a set of optimized reagents and standard operating procedures that allow basic and clinical research scientists to easily, conveniently and reproducibly isolate total proteins from human and non-human cell lines (attached and suspended), solid tissue and tumor biopsies for use in 2D-DIGE experiments (Fig 1).ToPI-DIGE is optimized for isolation of total proteins from cell lines (K-0010-C) and mammalian tissue (K-0010-T) for 2-Dimensional difference in gel-electrophoresis (2D-DIGE). Using the kit prevents protein breakdown and prevents additional buffer exchange steps that may lead to protein breakdown and lower yield.(Protease-, RNase- & DNAse- free) Order NowFig 1: 2D-DIGE image of Human Breast Cancer Cell Lines Proteins were isolated with ToPI-DIGE Kit. 50ug of protein from “reference”, “test” and “Internal Standards” were labeled with Cyanine dyes and loaded on the gel.Each Kit contains optimized buffer systems and consumables including:Buffer 1Buffer 2Buffer 3iTube AiTube B (Protease-, RNase- & DNAse- free)Validated SOP for total protein isolation from cell lines and tissue for 2D-DIGEK-0010-C10 (10 Assays) – $68.00K-0010-T10 (10 Assays) – $65.00 Click Here to Order on Amazon

isohelix的GeneFiX™唾液DNA微生物组收集装置

微生物研究表明，包括唾液，菌斑，皮肤和肠道在内的许多人类系统中菌群的平衡是健康的关键指标。

微生物组样品的传统收集和存储方法通常包括冷冻，这在冷链运输和存储中总是带来众所周知的后勤挑战，或者通常会使收集过程中的技术可行性变得复杂。

Isohelix已通过新型GeneFiX™唾液微生物组DNA收集设备解决了这些问题。

该设备具有专利的*¹防漏斗，无害缓冲液和简单的操作，非常适合远程或家庭测试。

坚固的样品管经过全面运输测试，达到95KPa，可以邮寄和存放。微生物DNA可以在室温下完全保存在预先填充的缓冲液中至少1年。稳定是即时的，缓冲液可防止细菌生长，因此样品在采样时可准确反映口腔微生物组。该设备和缓冲液不含任何痕量的人类或细菌DNA，因此研究人员可以对其结果的准确性充满信心。

样品管与许多高通量样品处理系统兼容，并包括独特的一维条码和人类可读代码，可实现精确的样品跟踪。