产品说明
Biological samples contain a complex mixture of proteins, which in living cells are localized in the membrane, nucleus and/or cytoplasm. Therefore, to identify the proteins that are predominant in each region, it is necessary to reduce the complexity of the total protein lysate by fractionating the lysate into membrane, nuclear and cytosol fractions. Reduction in sample complexity is also important when the detection of protein(s) that occur at low concentrations in any of the regions is desired. An ideal method should be reproducible, simple, convenient and cost effective.ITSI has a unique kit for fractionation and enrichment of proteins found in the nuclear, cytosol and membrane regions. The ITSIPrep ProFEK (K-0015) Protein Fraction and Enrichment Kit (ProFEK) provides a validated, fast, and cost-effective method to fractionate and enrich for membrane, cytosol, and nuclear proteins without ultra centrifugation. The optimized reagents and procedure provided with the kit allow for reproducible results and identification of unique region associated proteins. The extracted membrane, nuclear, and/or cytoplasmic protein fractions can be analyzed by mass spectrometry, SDS-PAGE (Fig 1), 2D-PAGE, 2D-DIGE (Fig 2), and western blotting.Fig 1: 1D-PAGE gel image of chicken heart protein lysate fractionated with ITSIPrep ProFEK kit into Nuclear (lane 1), Cytosol (lane 2) and Membrane (lane 3) protein fractions. A and B represent protein fractions obtained with 1x and 2x volume of solvent respectively, MWW is molecular weight marker and WT is Whole (unfractionated) tissue lysate.Benefits of using the ITSIPrep Protein Fractionation and Enrichment Kits:– Lowest cost/sample for selective enrichment of nuclear, cytosol and membrane proteins– No ultra centrifugation required– Reproducible results– Easy-to-follow protocolFig 2: Images showing proteins from human tissue lysate fractionated with ITSIPrep ProFEK kit to concentrate Nuclear, Cytosol and Membrane proteins prior to analysis by 2D-DIGEEach Kit contains optimized reagents and consumables including:ProFEK Buffer 1ProFEK Buffer 2 Order NowProFEK Buffer 3Disposable Pestle/Tube for sample homogenizationStandard protocolK-0015-20 (20 Assays) – $69.00 Click Here to Order on Amazon
isohelix的GeneFiX™唾液DNA微生物组收集装置
微生物研究表明,包括唾液,菌斑,皮肤和肠道在内的许多人类系统中菌群的平衡是健康的关键指标。
微生物组样品的传统收集和存储方法通常包括冷冻,这在冷链运输和存储中总是带来众所周知的后勤挑战,或者通常会使收集过程中的技术可行性变得复杂。
Isohelix已通过新型GeneFiX™唾液微生物组DNA收集设备解决了这些问题。
该设备具有专利的*¹防漏斗,无害缓冲液和简单的操作,非常适合远程或家庭测试。
坚固的样品管经过全面运输测试,达到95KPa,可以邮寄和存放。微生物DNA可以在室温下完全保存在预先填充的缓冲液中至少1年。稳定是即时的,缓冲液可防止细菌生长,因此样品在采样时可准确反映口腔微生物组。该设备和缓冲液不含任何痕量的人类或细菌DNA,因此研究人员可以对其结果的准确性充满信心。
样品管与许多高通量样品处理系统兼容,并包括独特的一维条码和人类可读代码,可实现精确的样品跟踪。
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