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Haematologic Technologies/Human Factor IXa, EGRck-active site blocked/HCIXA-EGR/100 µg

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Formulation20mMHepes,150mMNaCl,pH7.4Storage-80°CPurity>95%bySDS-PAGEActivityDeterminationFactorIXclottingassayShelfLife(properlystored)12monthsActivationofFactorXbyFactorIXaTheactivationoffactorXbytheintrinsicfactorXasecomplexisshown.TheintrinsicfactorXasecomplexconsistsofanenzyme,factorIXa,andacofactor,factorVIIIa,assembledonaphospholipidsurfaceinthepresenceofcalciumions.Theenzymecomplexproteolyticallycleavesa10,000molecularweightactivationpeptidefromtheNH2-terminaloftheheavychainoffactorX,thusexpressingtheactive-siteoffactorXa.SampleGelInformation:GelNovex4-12%Bis-TrisLoadHumanFactorIXa,1µgperlaneBufferMOPSStandardSeeBluePlus2;Myosin(191kDa),PhosphorylaseB(97kDa),BSA(64kDa),GlutamicDehydrogenase(51kDa),AlcoholDehydrogenase(39kDa),CarbonicAnhydrase(28kDa),MyoglobinRed(19kDa),Lysozyme(14kDa)Overview:FactorIXaisproducedfromitsinactiveprecursor,factorIX,viaproteolyticcleavagebyfactorXIaorthetissuefactor/factorVIIa/phospholipidcomplex.TheactivationresultsfromthecleavageoftwopeptidebondsinthefactorIXmolecule,releasinganactivationglycopeptidewithanapparentmolecularweightof10,000.TheheavychainoffactorIXa(Mr=28,000)containstheserineproteasecatalyticdomain,whilethelightchain(Mr=17,000)containsthemembranebindingdomain.FactorIXafunctionsasaserineproteaseinvolvedintheactivationofthezymogen,factorX,toformtheenzyme,factorXa.ThefactorIXaenzymaticactivityisgreatlyenhancedbyinclusionofitscofactor,factorVIIIa,inthepresenceofcalciumionsonaphospholipidsurface.FactorIXaisreADIlyinhibitedbyantithrombinIII,andthisinhibitionisgreatlyacceleratedbythepresenceofheparin.FactorIXaisnotinhibitedbyDFP.FactorIXaispreparedfromhighlypurifiedfactorIXbyactivationwithfactorXIa,asdescribedbyLindquistetal.(5).ThefactorIXaisfurtherpurifiedbygelfiltration,followedbyimmunoaffinitypurification.FactorIXaisalsoavailablewiththeactivesiteirreversIBLyblockedbythetripeptidechloromethylketone,EGRck,orbythefluorescentinhibitor,Dansyl-EGRck.Itissuppliedin50%(vol/vol)glycerol/H2Oforstorageat-20°C.PurityisassessedbySDS-PAGEanalysis.Activityisdeterminedinaone-stageclottingassay.Properties:LocalizationPlasmaModeofactionEnzymecomponentoftheFactorXasecomplexMolecularweight45,000(7)ExtinctioncoefficientE1%1cm,280nm=14.0(5)Structure2subunits,Mr=28,000and17,000(5),NH2-terminalgla-domain,twoEGFdomainsPost-translationalmodificationsoneb-hydroxyaspartate(3),twelveglaresidues(4)

Haematologic Technologies是一家通过ISO 9001：2015认证的公司，是一家主要制造商，专门从事旨在用于体外研究的高质量血浆蛋白的分离和表征的主要制造商。HT的重点是参与凝血级联反应的蛋白质，以及骨代谢的调节。HT产品系列包括150多种高度纯化且特性良好的蛋白质，包括酶原，酶，辅因子和抑制剂，以及单克隆和多克隆抗体的互补产品系列。还提供因子不足的血浆和定制的用于临床研究的定制采血管。提供的服务包括定制蛋白质纯化，蛋白质修饰，分析开发，合同制造和合同研究。