首页 > haemtech > Haematologic Technologies/Mouse Antithrombin III/MCATIII-5120/100µg

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Haematologic Technologies/Mouse Antithrombin III/MCATIII-5120/100µg

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MCATIII-5120

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Formulation50%(vol/vol)Glycerol/H2OMolecularWeightStorage-20°CPurity>95%bySDS-PAGECompoundAssayThrombininhibitionShelfLife(properlystored)12monthsChemicalFormulaDomainStructureofAntithrombinIIIAntithrombinIIIcontainsthreeintra-chaindisulfidebonds(-S-S-),acarbohydraterichdomain(CHO),anNH2-terminalheparinbindingdomain,andaCOOH-terminalserineproteasebindingdomain.SampleGelInformation:GelNovex4-12%Bis-TrisLoadHumanATIII,1µgperlaneBufferMOPSStandardSeeBluePlus2;Myosin(191kDa),PhosphorylaseB(97kDa),BSA(64kDa),GlutamicDehydrogenase(51kDa),AlcoholDehydrogenase(39kDa),CarbonicAnhydrase(28kDa),MyoglobinRed(19kDa),Lysozyme(14kDa)Overview:AntithrombinIII(ATIII)isasinglechainglycoproteinwithamolecularweightof58,000.Itisamemberoftheserpin(serineproteaseinhibitor)superfamilyandisconsideredtobethemostimportantinhibitorinthecoagulationcascade(1,2).ATIIIinhibitsawidespectrumofserineproteasesincludingthrombin,factorsIXa,XaandXIa,kallikrein,plasmin,urokinase,C1-esterase,andtrypsin.Themechanismofinhibitioninvolvestheformationofastable1:1complexbetweentheactivesiteoftheproteaseandthescissilebond(Arg385-Ser386)ofATIII.TheactivesiteserineofthrombinhasbeenshowntoformacovalentintermediatewiththeP1aminoacid(Arg385)ofATIII.TherateofinhibitionofserineproteasesbyATIIIisincreasedtovaryingdegreesbyheparin.InthecaseofbeingathrombininhibitororfactorXainhibitor,theinteractionwithATIIIisenhanced3ordersofmagnitudeinthepresenceofheparin.TheinteractionbetweenATIIIandheparininvolvesauniquesequenceofsulfatedandnon-sulfatedmonosaccharideunitsonheparin,andcriticallysineresiduesonATIII.ThebindingofATIIItoheparinoidstructuresonvascularendotheliumhasbeendemonstratedandshowntoenhancetheinhibitionoffactorsIXa,Xa,andthrombin.ATIIImayalsofunctioninthecomplementcascade.ThebindingofATIIItofluidphasecomplementattack-complexesinserahasbeendemonstrated.Inaddition,theSproteinofcomplement(aninhibitorofthemembraneattack-complex)interfereswiththeATIII/thrombininteraction.ATIIIispreparedfromfreshfrozenplasmabyheparin-agaroseaffinitychromatography(3).Thepurifiedproteinissuppliedin50%(vol/vol)glycerol/H2Oandshouldbestoredat-20°C.PurityisdeterminedbySDS-PAGEanalysis.Properties:LocalizationPlasmaPlasmaConcentration150µg/mlModeofactionSerineproteaseinhibitorMolecularweight58,000(3)ExtinctioncoefficientE1%1cm,280nm=6.2(4)IsoelectricPoint4.9-5.3(5)StructureSinglechain,threeintrachaindisulfidebonds(Cys8-Cys128,Cys21-Cys95,Cys239-Cys422)(4),10%a-helix,30-40%b-structure,50%randomcoil(5),scissilebond(Arg385-Ser386)Percentcarbohydrate9%(5,7)

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