CHIR99021 is a glycogen synthase kinase 3β (GSK3β) inhibitor with IC50 less than 10 nM(1,2). Compared to other GSK-3 inhibitors, CHIR-99201 does not exhibit cross-reactivity against cyclin-dependent kinases (CDKs) with 350-fold selectivity toward GSK-3β compared to CDKs. Inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ) (3). Together with mitogen-activated protein kinase inhibitor, CHIR99021 enables the self-renewal of embryonic stem cells(2).
CAS Number 252917-06-9
Chemical Formula C22H18Cl2N8
Chemical Name
6-(2-(4-(2,4-dichlorophenyl)-5-(4-methyl-1H-imidazol-2-yl)pyrimidin-2-ylamino)ethylamino)nicotinonitrile
Molecular Weight 465.34
Color Light yellow
QC Specifications MASS Spec Mouse ESC culture for toxicity
Shipping Information on blue ice
Storage -20°C
Stability Stable for up to 6 months from date of receipt when stored as directed.
Reference 1. Ring, D.B., et al. Selective glycogen synthase kinase 3 inhibitors potentiate insulin activation of glucose transport and utilization in vitro and in vivo (2003) Diabetes 52: 588-595.
2. Hall, R.K., et al. Regulation of phosphoenolpyruvate carboxykinase and insulin-like growth factor-binding protein-1 gene expression by insulin. The role of winged helix/forkhead proteins (2000) J Biol Chem 275: 30169-30175.
3. Finlay, D., et al. Glycogen synthase kinase-3 regulates IGFBP-I gene transcription through the Thymine-rich Insulin Response Element. BMC Mol Biol 5: 15.
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第一次养胚胎干细胞,复苏的时候复苏到六孔板中的其中一个孔中,结果发现细胞很多,于是换了两个6cm皿养,结果越养越少。很纳闷。请教了几位蚂蚁淘的前辈们,于是乎有几个问题还是在心中,希望还能在这里能得到答复。
1。培养细胞的时候是一定要在六孔板中吗?那一般复苏细胞的时候复苏到几个孔中呢?
2.细胞的密度是大概多少呢?
3.我用的基质胶,说明书上是1;80,结果在孵箱放了一小时后,发现没有想象的胶凝固的样子,没有像果冻那样。反而还是液体状的,这是能用还是不能用呢?
4.如果基质胶一开始就铺好,等到传代的时候再铺,中间每天都在换液,不容易污染吗?
5。刚复苏的细胞,一直漂浮在培养液里,是慢慢地细胞才会贴在基质胶上么,然后才会慢慢在基质胶上生长么?
全能干细胞具有能发育成各种组织器官的完整个体的潜能的细胞,如胚胎干细胞。多能干细胞没有发育成完整个体的能力,但具有分化出多种细胞组织的能力,主要在器官再生、修复和疾病治疗方面极具应用价值,多能干细胞还在研究当中,过去认为多能干细胞只能从人胚胎中获得,现在已能用体细胞转化为多能干细胞。
单能干细胞只能向一种类型或密切相关的两种类型的细胞分化,如上皮组织基底层的干细胞、肌肉中的成肌细胞。
一般细胞没有自我复制能力,而且也不会分化。
原代培养效果很好,第四天便成典型梭形鱼群状生长;第六天传代
但传代后细
胞形态发生改变,属于正常吗,请大神知道,初次培养;万分感谢
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