Product Name | AnaTagᵀᴹHiLyteᵀᴹFluor 647 Microscale Protein Labeling Kit *Ultra Convenient* |
Size | 1 kit |
Catalog # | AS-72050 |
US$ | $235 |
HiLyte Fluor 647 acid, SE (Ex/Em=647 nm/ 679 nm) is an excellent fluorescent labeling dye that generates the protein conjugates that are slightly red-shifted compared to those of Cy® 5 dyes, resulting in an optimal match to filters designed for Cy®5 dyes. However, total fluorescence of the secondary antibody conjugates of HiLyte Fluor 647 dyes is significantly higher than that of Cy®5 conjugates supplied by other companies. Also, unlike Cy®5 dyes, HiLyte Fluor 647 dyes have very little change in absorption or fluorescence spectra when conjugated to most proteins, oligonucleotides and nucleic acids, thus yielding greater total fluorescence at the same degree of substitution. The HiLyte Fluor 647-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. Additionally, our in-house data indicated that HiLyte Fluor 647 is superior to Cy®5 in fluorescence polarization-based assays. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte Fluor 647-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG. Kit Size: 3 labeling reactions (3 x 200 µg protein) | |
Detailed Information | DatasheetMaterial Safety Data Sheets (MSDS) |
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第一次养胚胎干细胞,复苏的时候复苏到六孔板中的其中一个孔中,结果发现细胞很多,于是换了两个6cm皿养,结果越养越少。很纳闷。请教了几位蚂蚁淘的前辈们,于是乎有几个问题还是在心中,希望还能在这里能得到答复。
1。培养细胞的时候是一定要在六孔板中吗?那一般复苏细胞的时候复苏到几个孔中呢?
2.细胞的密度是大概多少呢?
3.我用的基质胶,说明书上是1;80,结果在孵箱放了一小时后,发现没有想象的胶凝固的样子,没有像果冻那样。反而还是液体状的,这是能用还是不能用呢?
4.如果基质胶一开始就铺好,等到传代的时候再铺,中间每天都在换液,不容易污染吗?
5。刚复苏的细胞,一直漂浮在培养液里,是慢慢地细胞才会贴在基质胶上么,然后才会慢慢在基质胶上生长么?
全能干细胞具有能发育成各种组织器官的完整个体的潜能的细胞,如胚胎干细胞。多能干细胞没有发育成完整个体的能力,但具有分化出多种细胞组织的能力,主要在器官再生、修复和疾病治疗方面极具应用价值,多能干细胞还在研究当中,过去认为多能干细胞只能从人胚胎中获得,现在已能用体细胞转化为多能干细胞。
单能干细胞只能向一种类型或密切相关的两种类型的细胞分化,如上皮组织基底层的干细胞、肌肉中的成肌细胞。
一般细胞没有自我复制能力,而且也不会分化。
原代培养效果很好,第四天便成典型梭形鱼群状生长;第六天传代
但传代后细
胞形态发生改变,属于正常吗,请大神知道,初次培养;万分感谢
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