Cell Transfection Reagents
Catalog# | size | Price | Quantity | |
---|---|---|---|---|
31-00110 | 1ml (250 Tfx) | $180.00 | ||
31-00120 | 1ml (250 Tfx) | $180.00 | ||
31-00130 | 1ml (250 Tfx) | $220.00 |
Epoch Life Science"s GenCarrier TM transfection reagents apply a proprietary Lipoplex-enhancing mechanism to obtain the best transfection outcome - highest transgene expression and lowest cytotoxicity.
Epoch Life Science is determined to maintain the highest standards and deliver the highestquality products and services. Every batch is strictly tested to meet the requirementsof cell-culture grade bioproducts.
GenCarrier-1TM DNA transfection reagent - works in serum containing media
GenCarrier-1TM has been used successfully to transfect DNA into HeLa, CHO-K1, COS, LNCaP,NIH3T3, U937, SF-9, S2 (Schneider - Drosophila), 293, T-24, C2C12, PANC-1, primary human keratinocytes,primary aortic smooth muscle, primary rabbit myoblasts, human bone marrow endothelial cells (HBMEC),and other cell lines. No significant toxicity was observed at working concentrations on the cell lines tested.Enough reagent is provided for approximately 250 transfections in 35-mm plates per package.
PC12 cells transfected withpCMV-GFP using GenCarrier-1(upper 2 pictures) and lipofectamine 2000 (lower 2 pictures)
GenCarrier-2TM DNA transfection reagent -works in primary cell culture GenCarrier-2TM has been used successfully to transfect DNA into COS, HepG2, PC12, NIH3T3, Jurkat, PC-12, HUVEC, primary human pancreatic islet cells, primary rat and hamsterhepatocytes, primary rat cardiomyocytes, primary male rat germ cells, primary chick retinal neurons,primary rat fibroblasts, primary human keratinocytes, and tracheobroncial cells. No significant toxicitywas observed at working concentrations on the cell lines tested.Enough reagent is provided for approximately 250 transfections in 35-mm plates per package.
Human primary pancreatic cells transfected with pCMV-bGal using GenCarrier-2 showed >50% transfection efficiency
RNAiCarrierTM siRNA transfection reagent
RNAiCarrierTM has been used successfully to transfect siRNA into HEK293, HuH-7 (human hepatoma), B16 melanoma, CHO-K1, COS-1, COS-7, NIH3T3, SF-9, S2, primary human keratinocytes, primary rat aortic smooth muscle (WKY3M), and other cell lines. No significant toxicity was observed at working concentrations used on the cell lines tested.
Enough reagent is provided for approximately 250 transfections in 35-mm plates per package.
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由于质粒的不兼容性,拥有同种复制子的质粒不能在同一细胞内稳定共存,经过几代的复制,会质粒丢失,所以并不是任何两个或两个以上质粒都可以在同一细胞内稳定存在,但是可以同时进入.
稳定转染的细胞株,就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
如果经费充足的话可以找公司包装病毒,如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。
先提取RNA,反转录成cDNA,然后根据目的基因设计PCR引物,通过半定量RT-PCR确定目的基因表达.
正因为检测的是mRNA,所以要先反转录成cDNA才能PCR.
2.将构建完成的载体与慢病毒包装质粒混合,共转染靶细胞
3.收集病毒液
4.用病毒液感染靶细胞
5.用载体上带的抗生素进行筛选,如果没有,可以用无限稀释法
6.获得稳转株
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