Amount : | Non Profit |
The GATA3 Leeporter™ Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the GATA3 response element, so that the cell line is designed to measure the transcriptional activity of GATA3. As a zinc-finger transcription factor, GATA3 (GATA-binding protein 3) plays a critical role in early and late T cell differentiation, which regulates Th1/Th2 differentiation. GATA3 has been shown to induce Th2 differentiation and repress Th1 differentiation. GATA3 is also known to promote the secretion of IL-4, IL-5 and IL-13 from Th2 cells. The GATA3 induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.
Content : | Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO. |
Storage condition : | Immediately upon receipt, store in liquid nitrogen. |
Application:
- Monitor the GATA3 signaling pathway activity.
- Screen for activators or inhibitors of the GATA3 signaling pathway.
Culture conditions:
A. Response of GATA3 Leeporter™ – HEK293 cells to phorbol 12-myristate 13-acetate (PMA).
LIMITED USE RESTRICTIONS:
THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
By use of this product, user agrees to be bound by the terms of this limited use statement.
This product issolely for Internal Research Purposesandnot for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.
Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
There are currently no product reviews Write a review on this product! |
Customers who purchased this product also purchasedTNF-beta Leeporter™ Lucifera...
TNF-beta Leeporter™ Lucifera...
TNF-alpha Leeporter™ Lucifer...
TNF-alpha Leeporter™ Lucifer...
MIP-2 Leeporter™ Luciferase ...
MIP-2 Leeporter™ Luciferase ...
AP-1 Leeporter™ Luciferase R...
IL-8 Leeporter™ Luciferase R...
NF-kB Leeporter™ Luciferase ...
Human Peripheral Blood CD19/CD...
Human Peripheral Blood CD19 B ...
Human Peripheral Blood CD14 Mo...
Human Peripheral Blood CD8 Cyt...
Human Peripheral Blood CD8/CD4...
TNF-beta Leeporter™ Lucifera...
TNF-beta Leeporter™ Lucifera...
TNF-alpha Leeporter™ Lucifer...
TNF-alpha Leeporter™ Lucifer...
MIP-2 Leeporter™ Luciferase ...
MIP-2 Leeporter™ Luciferase ...
AP-1 Leeporter™ Luciferase R...
IL-8 Leeporter™ Luciferase R...
NF-kB Leeporter™ Luciferase ...
Human Peripheral Blood CD19/CD...
Human Peripheral Blood CD19 B ...
Human Peripheral Blood CD14 Mo...
Human Peripheral Blood CD8 Cyt...
Human Peripheral Blood CD8/CD4...
Most viewed Products
Recombinant Human Thioredoxin-Like ...
Monoclonal antibody to Human PD-L1 ...
Monoclonal Antibody to Caspase-3 (P...
Polyclonal Antibody to Beta actin
Monoclonal Antibody to GAPDH (Clone...
Monoclonal Antibody to Human IL-1be...
Monoclonal antibody to B7-H4 (Clone...
Polyclonal Antibody to Beta Tubulin
Peroxidase conjugated Goat anti Mou...
Monoclonal Antibody to EpCAM (CD326...
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
由于质粒的不兼容性,拥有同种复制子的质粒不能在同一细胞内稳定共存,经过几代的复制,会质粒丢失,所以并不是任何两个或两个以上质粒都可以在同一细胞内稳定存在,但是可以同时进入.
稳定转染的细胞株,就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
如果经费充足的话可以找公司包装病毒,如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。
先提取RNA,反转录成cDNA,然后根据目的基因设计PCR引物,通过半定量RT-PCR确定目的基因表达.
正因为检测的是mRNA,所以要先反转录成cDNA才能PCR.
2.将构建完成的载体与慢病毒包装质粒混合,共转染靶细胞
3.收集病毒液
4.用病毒液感染靶细胞
5.用载体上带的抗生素进行筛选,如果没有,可以用无限稀释法
6.获得稳转株
暂无品牌问答