Product Name | HCV NS3/4A protease genotype 1b, recombinant |
Size | 5 µg |
Catalog # | AS-61017-5 |
US$ | $247 |
The NS3 protease of hepatitis C virus (HCV) is responsible for the cleavage at the NS3/NS4A, NS4A/NS4B, NS4B/NS5A, and NS5A/NS5B sites of the nonstructural protein. It is essential for viral replication and the formation of infectious viral particles, and thus has been considered as one of the most attractive targets for anti-HCV therapy. Recombinant HCV NS3/4A protease is highly active and can be used with SensoLyte® 490 or 520 HCV Protease Assay Kits (Cat# 71126, 71145) to screen for anti-HCV protease drugs. | |
Detailed Information | DatasheetMaterial Safety Data Sheets (MSDS)Poster |
Storage | Store at -80°C. Avoid multiple thaw-freeze cycles. |
References | 1. R. Bartenschlager, R. et al. J.Virol. 67, 3835-3844 (1993).2. Eckart, MR. et al. Biochem.Biophys.Res.Commun. 192, 399-406 (1993).3. Landro, JA. et al. Biochemistry 36, 9340-9348 (1997).4. Kim, JL. et al. Cell 87, 343-355 (1996).5. Love, RA. et al. Cell 87, 331-342 (1996). |
Product Citations | Ma, C. et al. (2009). HCV Protease Inhibitory, Cytotoxic and Apoptosis-Inducing Effects of Oleanolic Acid Derivatives. J Pharm Pharmaceut Sci 12, 243.Phuong, DT. et al. (2009). Inhibitory effects of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. Phytother Res 23, 582.of antrodins A-E from Antrodia cinnamomea and their metabolites on hepatitis C virus protease. Phytother. Res. 23, 582.Yu, X. (2009). Development of a cell-based hepatitis C virus (HCV) infection FRET assay for high throughput antiviral compound screening. Antimicrob Agents Chemo doi:10.1128/AAC.00495-09.Ma, C. et al. (2007). Triterpenes from Cynomorium songariciumanalysis of HCV protease inhibitory activity, quantification, and content change under the influence of heating. J Nat Med 63, 9. |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
由于质粒的不兼容性,拥有同种复制子的质粒不能在同一细胞内稳定共存,经过几代的复制,会质粒丢失,所以并不是任何两个或两个以上质粒都可以在同一细胞内稳定存在,但是可以同时进入.
稳定转染的细胞株,就是转染后质粒可以稳定整合到基因组上不会因细胞分裂而丢失。区别于质粒瞬时转染不能长时间保留质粒在细胞里。
如果经费充足的话可以找公司包装病毒,如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。
先提取RNA,反转录成cDNA,然后根据目的基因设计PCR引物,通过半定量RT-PCR确定目的基因表达.
正因为检测的是mRNA,所以要先反转录成cDNA才能PCR.
2.将构建完成的载体与慢病毒包装质粒混合,共转染靶细胞
3.收集病毒液
4.用病毒液感染靶细胞
5.用载体上带的抗生素进行筛选,如果没有,可以用无限稀释法
6.获得稳转株
暂无品牌问答