Product Name | Biotin - ACTH (1 - 39), humanBiotin - SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF |
Size | 0.5 mg |
Catalog # | AS-23968 |
US$ | $232 |
Purity | % Peak Area By HPLC ≥ 95% |
This C-terminally labeled biotin ACTH (1-39) has been used in ELISA assays. Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a cleavage product from a larger precursor proopiomelanocortin (POMC). This 39 amino acid-peptide hormone is produced in the anterior pituitary gland upon stimulation by the corticotropin releasing hormone from the hypothalamus in response to stress. It stimulates the secretion of steroid hormone, specifically glucocorticoids in the adrenal cortex by acting through a cell membrane receptor (ACTH-R). In mammals, the action of ACTH is limited to those areas of the adrenal cortex in which the glucocorticoid hormones cortisol (hydrocortisone) and corticosterone are formed. ACTH has little control over the secretion of aldosterone, the other major steroid hormone from the adrenal cortex. | |
Detailed Information | Material Safety Data Sheets (MSDS) |
Storage | -20°C |
References | Moreno-Guzmán, M. et al. Biosensors Bioelectronics 35, 82 (2012). doi: 10.1016/j.bios.2012.02.015Stewart, PM. et al. Clin Endocrinol 40, 199 (1994)Elias, LL. and AJ. Clark, Braz J Med Biol Res 33, 1245 (2000)Latronico, AC. Braz J Med Biol Res 33, 1249 (2000) |
Molecular Weight | 4767.6 |
Biotin-SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF | |
Sequence(Three-Letter Code) | Biotin - Ser - Tyr - Ser - Met - Glu - His - Phe - Arg - Trp - Gly - Lys - Pro - Val - Gly - Lys - Lys - Arg - Arg - Pro - Val - Lys - Val - Tyr - Pro - Asn - Gly - Ala - Glu - Asp - Glu - Ser - Ala - Glu - Ala - Phe - Pro - Leu - Glu - Phe - OH |
Product Citations | Moreno-Guzmán, M. et al. (2012). Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors. Biosensors Bioelectronics 35, 82. doi: 10.1016/j.bios.2012.02.015. |
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1.少量表达时用玻璃珠振荡的方法破碎细胞,跑胶时蛋白量总是很低,有什么好的方法进行少量酵母细胞的破碎吗?
2.好像有一种试剂加到玻璃珠振荡破碎的酵母细胞样品中来镜鉴细胞是否破碎的,是什么试剂啊?
多谢指点!
另外还想问一下,还有其他可行的破碎方法吗
谢谢
大家请赐教!
我怀疑溶菌酶有问题,因为其消化的30min内菌液几乎没大变化,而我曾看到别人用溶菌酶消化时菌液变的挺粘稠。
今下午再做时还是没什么变化,但发现溶菌酶在冰上静置2-3h后竟然沉淀下来了,而且上清和下面的白色溶菌酶分界明显,是我的溶菌酶配的有问题吗?出现这种情况溶菌酶还能用吗?我是-20度保存的。
下一步怎么处理菌液呢?
请高手多多指教!
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