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RayBiotech/Human Cytokine Array Q1/8 Sample Kit/QAH-CYT-1-1
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RayBiotech/Human Cytokine Array Q1/8 Sample Kit/QAH-CYT-1-1
品牌 / 
RayBiotech
货号 / 
QAH-CYT-1-1
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数    量:
免费咨询热线
4000-520-616

Antigen Information

Gene Symbols:
CCL2 | CCL3 | CCL4 | CCL5 | CSF2 | CXCL1 | CXCL2 | CXCL3 | CXCL8 | IFNG View more Hide

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF
  • Gene Symbol: CSF2
  • Gene ID: 1437
  • Accession #: P04141
  • ELISA Kit: RayBio Human GM-CSF ELISA
GRO alpha/beta/gamma
  • Gene Symbol: CXCL1/CXCL2/CXCL3
  • Gene ID: 2919/2920/2921
  • Accession #: P09341/P19875/P19876
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
  • ELISA Kit: RayBio Human IFN-gamma ELISA
IL-1 alpha(IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 3552
  • Accession #: P01583
  • ELISA Kit: RayBio Human IL-1 alpha ELISA
IL-1 beta(IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 3553
  • Accession #: P01584
  • ELISA Kit: RayBio Human IL-1 beta ELISA
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
  • ELISA Kit: RayBio Human IL-10 ELISA
IL-12 p70
  • Gene Symbol: IL12A
  • Gene ID: 3592
  • Accession #: P29459
  • ELISA Kit: RayBio Human IL-12 p70 ELISA
IL-13
  • Gene Symbol: IL13
  • Gene ID: 3596
  • Accession #: P35225
  • ELISA Kit: RayBio Human IL-13 ELISA
IL-2
  • Gene Symbol: IL2
  • Gene ID: 3558
  • Accession #: P60568
  • ELISA Kit: RayBio Human IL-2 ELISA
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
  • ELISA Kit: RayBio Human IL-4 ELISA
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
  • ELISA Kit: RayBio Human IL-5 ELISA
IL-6
  • Gene Symbol: IL6
  • Gene ID: 3569
  • Accession #: P05231
  • ELISA Kit: RayBio Human IL-6 ELISA
IL-8(CXCL8)
  • Gene Symbol: IL8
  • Gene ID: 3576
  • Accession #: P10145
  • ELISA Kit: RayBio Human IL-8 ELISA
MCP-1(CCL2)
  • Gene Symbol: CCL2
  • Gene ID: 6347
  • Accession #: P13500
  • ELISA Kit: RayBio Human MCP-1 ELISA
MIP-1 alpha(CCL3)
  • Gene Symbol: CCL3
  • Gene ID: 6348
  • Accession #: P10147
  • ELISA Kit: RayBio Human MIP-1 alpha ELISA
MIP-1 beta(CCL4)
  • Gene Symbol: CCL4
  • Gene ID: 6351
  • Accession #: P13236
  • ELISA Kit: RayBio Human MIP-1 beta ELISA
MMP-9
  • Gene Symbol: MMP9
  • Gene ID: 4318
  • Accession #: P14780
  • ELISA Kit: RayBio Human MMP-9 ELISA
RANTES(CCL5)
  • Gene Symbol: CCL5
  • Gene ID: 6352
  • Accession #: P13501
  • ELISA Kit: RayBio Human RANTES ELISA
TNF alpha
  • Gene Symbol: TNF
  • Gene ID: 7124
  • Accession #: P01375
  • ELISA Kit: RayBio Human TNF-alpha ELISA
VEGF-A
  • Gene Symbol: VEGFA
  • Gene ID: 7422
  • Accession #: P15692
  • ELISA Kit: RayBio Human VEGF-A ELISA

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Cytokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser ScannersDon"t have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Chen T., Li H., Lin J., et al. Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array. Tuberculosis (Edinb). 2016 Mar;97:108-17. doi: 10.1016/j.tube.2015.12.007. Species: HumanSample type: Serum
  2. Yang J., Fang P., Yu D., et al. Chronic Kidney Disease Induces Inflammatory CD40+ Monocyte Differentiation via Homocysteine Elevation and DNA Hypomethylation. Circ Res. 2016 Nov 11;119(11):1226-1241.Species: HumanSample type: Serum
  3. Chen S., Zha X., Shi L., et al. Upregulated TCR? improves cytokine secretion in T cells from patients with AML. J Hematol Oncol. 2015; 8: 72. Published online 2015 Jun 18. doi: 10.1186/s13045-015-0170-0Species: HumanSample type: Conditioned Media
  4. Zhan Y., Zou S., Hua F., Li F., Ji L., Wang W., Ye Y., Sun L., Chen H., Cheng Y. High-dose dexamethasone modulates serum cytokine profile in patients with primary immune thrombocytopenia. Immunology Letters Volume 160, Issue 1, July 2014, Pages 33-38Species: HumanSample type: Serum
  5. Wu H., et al. Upregulation of innate immune responses in a T cell/histiocyte-rich large B cell lymphoma patient with significant autoimmune disorders mimicking systemic lupus erythematosus. Annals of Hematology (2014) 93:353-354Species: HumanSample type: Plasma
  6. Jiang, Weidong, Ruo-Pan Huang, and Ruochun Huang. "Protein Expression Profiling by Antibody Array Analysis with Use of Dried Blood Spot Samples on Filter Paper." Journal of Immunological Methods. ScienceDirect, 25 Nov. 2013. Web.Species: HumanSample type: Serum
  7. KrÃ?nkel, Nicolle, et al. "Novel insights into the critical role of bradykinin and the kinin B2 receptor for vascular recruitment of circulating endothelial repair-promoting mononuclear cell subsets: alterations in patients with coronary disease."?Circulation?(2012): CIRCULATIONAHA-112.Species: HumanSample type: Conditioned Media
  8. LÃ?nne, M., Lavrentieva, A., Walter, J. G., & Kasper, C. (2013). Analysis of oxygen-dependent cytokine expression in human mesenchymal stem cells derived from umbilical cord.?Cell and tissue research,?353(1), 117-122.Species: HumanSample type: Cell Lysate
  9. Logan, Patrick, Julia Burnier, and Miguel N. Burnier Jr. "Vascular endothelial growth factor expression and inhibition in uveal melanoma cell lines."?ecancermedicalscience?7 (2013).Species: HumanSample type: Conditioned Media
  10. Varnum S., Springer D., Chaffee M., et al. The effects of low-dose irradiation on inflammatory response proteins in a 3D reconstituted human skin tissue model. Radiat Res. 2012 Dec;178(6):591-9. doi: 10.1667/RR2976.1.Species: HumanSample type: Tissue Lysate
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It's user friendly and worked really well for me.
University of Colorado Anschutz Medical School
This kit has been used to quantify the cytokines in the serum from patients in the following paper: Circ Res. 2016 Nov 11;119(11):1226-1241. This assay is very sensitive.
Temple University
Directions easy to follow and they provide free scanning services. I also recommend the software.
University of Colorado Anschutz Medical Campus
Definitely one of the better cytokine arrays I've worked with.
Washington University in St. Louis
Instructions were very clear and straightforward; it was a relatively simple process for a first-time user. Plenty of buffers and diluents included, and free analyzing by Ray-biotech if you don't have a compatible scanner accessible.
Washington University in St. Louis
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