Product Name | SensoLyte ® 520 Factor Xa Assay Kit *Fluorimetric* |
Size | 1 kit |
Catalog # | AS-72208 |
US$ | $526 |
Factor Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits. FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex. FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways and is the rate-limiting step in the propagation of thrombin generation. In the presence of Ca2+ ions, FXa forms prothrombinase with factor Va on the phospholipid membrane of the activated platelets. FXa has emerged as an attractive target for drug discovery for thromboembolic diseases. The SensoLyte® 520 Factor Xa Assay Kit provides a convenient assay for high throughput screening of FXa inhibitors or continuous assay of enzyme activity. It detects FXa activity using a 5-FAM/QXL® 520 fluorescence resonance energy transfer (FRET) peptide. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520.Upon cleavage into two separate fragments by FXa, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission wavelengths = 490 nm/520 nm. With excellent fluorescence quantum yield and longer wavelength, 5-FAM shows less interference from autofluorescence of test compounds and cellular components. The assays are performed in a convenient 96-well microplate format. Kit size: 100 assays | |
Detailed Information | DatasheetMaterial Safety Data Sheets (MSDS) |
Product Citations | Lagos, F. C. et al. (2017). Novel FXa Inhibitor Identification through Integration of Ligand- and Structure-Based Approaches molecules 22 1588, doi:10.3390/molecules22101588 |
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POT -HG POT-LIN POT-LOG 三个都可调。其中POT-HG电阻上显示调整的%数这个估计需要详细的说明才弄的了去硬之城看看吧或许有人会。
而方波是一种数字信号,其电信号在一应时间内保持持续稳定的点击,并在点击后瞬间消失,对细胞的损伤较小,转化效率较高。
主要是用于酵母,细菌等的转化.
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