- SynonymKDR,CD309,FLK1,VEGFR,VEGFR2
- SourceMouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (VE2-M5258) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 762 (Accession # P35918-1).Predicted N-terminus: Ala 20Request for sequence
- Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 110.1 kDa. The protein migrates as 120-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 0.1 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE2-M5258) with a linear range of 1-31 ng/mL (QC tested).
Immobilized ActiveMax® Mouse VEGF120, Tag Free (Cat. No. VE0-M4211) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE2-M5258) with a linear range of 1-31 ng/mL (Routinely tested).
Immobilized ActiveMax® Mouse VEGF164, Tag Free (HPLC-verified) (Cat. No. VE4-M4216) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE2-M5258) with a linear range of 2-31 ng/mL (Routinely tested).
- BackgroundKinase insert domain receptor (KDR) is also known as CD309, FLK1, VEGFR, VEGFR2, andis one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo). In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi"s sarcoma lesions.
- References
- (1)Holmes K,et al., 2007, Cell Signal. 19 (10): 2003–2012.
- (2)Stuttfeld E, et al., 2009,IUBMB Life 61 (9): 915–22.
- (3)Fujita, N, et al., 2008, Biochem Biophys Res Commun, 372(2):367-72.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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求问酶切体系为10ul,内切酶1ul,37℃水浴45min,不知道DNA产物是否完全被切开了?
所用内切酶信息如下
配后是否应该分装,因为不能反复冻融?
多谢!
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【】可以这样输入,智能ABC,输入“v1”,后翻3页,就可以看见了,或用紫光拼音中文输入法,输入方括号就是粗体的中括号,实在不行请复制本公告中【】
同一管dna有的酶可以切开,比如dra1,ecoR1,Hind111
有的酶又完全不能切动,如BamH1,sac1,xba1,xho1,pst1
请问高手这是什么原因??
我做的双酶切反应,酶分别是宝生物的BamHI和XhoI,双酶切体系是:载体及目的片段分别是30ul,通用buffer4ul,XhoI、BamHI各2ul,无核酶水2ul,总体积40ul。载体和目的片段都是经37度酶切6h。载体上面这两个酶切位点的距离是6个碱基,目的片段是由pcr反应获得的,从puc19中p出来的,两端带有这两种酶的酶切位点,酶切完成后,做连接反应,体系如下:目的片段(全长1441bp)4ul,无核酶水3ul,10*T4DNA连接酶缓冲液1ul,载体1ul,T4DNA连接酶1ul,总体积10ul。16度过夜。之后摇菌送沉菌测序结果回来,送了5管一个都不对。重复实验两次还是不对!
之后改为先用BanHI单切载体,体系如下:载体17ul,酶1ul,buffer2ul,总体积20ul,30度切6小时后,Promega纯化试剂盒直接纯化,完了之后再用XhoI,37度酶切6小时,体系如上,再次用promega纯化试剂盒纯化,目的片段用双酶切体系酶切,之后做连接反应,之后铺板,挑取菌落共10个,摇菌13小时,取菌液做pcr鉴定,结果10个菌落全是引物2聚体,跑出来的条带都是100bp左右。
各位老师这是怎么回事呢?为什么连接不上呢?小弟先谢过各位大哥了!
感谢赐教~!
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