稀释限制性内切酶时,建议使用稀释缓冲液(A,B,C)。建议临用前稀释且稀释终浓度不要小于1,000units/ml。目录及说明书中标注了每一种内切酶相应的稀释兼容性。 注:以下稀释液不适用于耐热DNA聚合酶。欲了解稀释耐热DNA聚合酶的相关情况,请参见相应章节。 稀释缓冲液成份: 稀释液A:50mMKCl,10mMTris-HCl,0.1mMEDTA,1mMDTT,200µg/mlBSA和50%甘油(pH7.4@25°C)。 稀释液B:300mMNaCl,10mMTris-HCl,0.1mMEDTA,1mMDTT,500µg/mlBSA和50%甘油(pH7.4@25°C)。 稀释液C:250mMNaCl,10mMTris-HCl,0.1mMEDTA,1mMDTT,0.15%TritonX-100,200µg/mlBSA和50%甘油(pH7.4@25°C)。 *表示稀释时需要添加MgCl2至终浓度10mM 可用稀释缓冲液A进行稀释的酶: AATIIAccIAcc65IAciIAfeIAflIIAhdIAlwIAlwNIApaIApaLIApoIAscIAvaIAvaIIBaeIBamHIBanIBanIIBbvIBbvCIBccIBceAIBcgIBciVIBclIBfrBIBfuAIBfuCIBglIIBlpIBme1580IBmtIBpu10IBpuEIBsaAIBsaBIBsaHIBsaJI BsaWIBseRIBsiEIBsiWIBslIBsmIBsmFIBsoBIBsp1286IBspCNIBspDIBspEIBspHIBsrBIBsrDIBsrGIBssHIIBssKIBstAPIBstBIBstEIIBstF5IBstNIBstUIBstXIBstYIBsu36IBtgIBtgZIBtsIClaIDdeIDraIDrdIEaeIEarIEciIEcoNIEco0109IEcoRV FatIFauIFnu4HIFokIHaeIIHaeIIIHgaIHhaIHinfIHinP1IHpaIHpaIIHpy99IHpy188IHpy188IIIHpyCH4IIIHpyCH4IVKpnIMboIMboIIMcrBcMfeIMluIMlyIMseIMslIMspINaeIN.AlwINarIN.BbvCIAN.BbvCIBN.BstNBINciINcoINdeINgoMIVNruINsiI NspIPacIPaeR7IPciIPflFIPflMIPhoIPleIPmeIPmlIPshAIPsiIPspGIPspOMIPvuIIRsaISacISacIISalISau3AISau96ISbfIScaIScrFISexAISgrAISmaISmlISnaBISpeIStuIStyITsp45ITsp509I*XbaIXcmIXhoIXmaIXmnIZraI 可用稀释缓冲液B进行稀释的酶: 可用稀释缓冲液C进行稀释的酶:AclIAflIII*AleIAluIAcuIApekIAseIAsiSIAvrIIBbsIBbvIBfaIBfuAIBglIBmrIBpmI* BsaIBsaXIBseYIBsgIBsiHKAIBsmAIBsmBIBspMI*BsrI*BssSIBstZ17ICac8ICviAIIDpnIDpnIIDraIII EagIFseIHincIIHindIIIHphIHpyCH4VI-CeuII-SceIKasI*MmeIMnlIMscIMspA1I*MwoINlaIIINlaIV PI-PspIPI-SceIPpuMIPvuISfaNISfcISfoISphIStyD4ISwaITaqITliITth111ITseITspRI AgeIBmgBIBsrFIEcoRI FspINheINotI PstIRsrIISapI SfiISspITfiI