Recombinant Human His6-UBE2G1 Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-700
Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UBE2G1
Ubiquitin-conjugating Enzyme E2G 1 (UBE2G1) is a 170 amino acid (aa) member of the Ubiquitin-conjugating (E2) enzyme family with a predicted molecular weight of 19.5 kDa. The human protein shares 100% aa sequence identity with the mouse and rat orthologs. UBE2G1 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). The expression level of UBE2G1 is downregulated when miR-199a is overexpressed, suggesting that UBE2G1 mRNA is targeted by miR-199a (2). UBE2G1 has been reported to function with the CRL4(Cdt2) Ubiquitin ligase (E3) to promote the poly-ubiquitination and degradation of Cdt1 (3). Reduced expression of UBE2G1 in medulloblastoma tumors has also been reported, suggesting that it may act as a tumor suppressor (4). This protein contains an N-terminal 6-His tag.
- Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
- Haghikia, A. et al. (2011) Eur. Heart J. 32:1287.
- Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
- Cvekl, A. Jr. et al. (2004) Eur. J. Cancer 40:2525.
Citation for Recombinant Human His6-UBE2G1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndromeAuthors: KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES FischerElife, 2018;7(0):.Species: HumanSample Types: ProteinApplications: Ubiquitination
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1.怎么看两个反应-即目标基因和内参基因是否具有相同的扩增效率?
2.每次PCR反应的扩增情况都有差异,多次反应的扩增效率岂不是都不一样了吗?
3.如何设计才能让相对定量更可行?
由于对定量PCR的接触不多,希望各位高手多多帮助!谢谢!
所以想请大家给推荐一个效果好的最好是国产的质粒提取试剂盒。
多谢!
这些染料的结构式基本是保密的。有些地方能查到,象维基上就有SYBR Green的结果式,但谁也不能肯定是否是正确的。
1.熔融温度为75,74,73度的样品是否是同一个产物?熔融温度为56度的是不是没有扩增产物?
2.为什么相同的标准品不同反应CT值差别这么大?是不是荧光染料降解?我用的是试剂盒中的扩增酶,还没用几次。
3.扩增曲线的形态是否说明标准品已经有降解?
4.另外,我原来做CDNA梯度稀释做的挺好,可是这个直接是RNA为标准品,我用一步法进行QRT-PCR。标准品梯度稀释线性效果不稳定,有的时候好,有的时候不好。请假大家有没有好的建议?
附件中有扩增曲线和熔融曲线,请大家帮忙分析,谢过了!
新建MicrosoftOfficeWord文档.docx(46.18k)
既说像坐标画曲线曲线应纵轴该荧光强度吧轴数值变化代表荧光强度变化荧光强度变化代表钙离浓度变化 我觉直接用荧光强度值变化代替钙离浓度变化研究意义该钙离浓度变化吧
非要荧光强度值换算钙离浓度需要做列工作
1 取标准钙离浓度染色用共聚焦显微镜测荧光强度
2 标准品需要同像环境主要曝光间相同激光波及能量
3 标准品要用同浓度荧光染料
才能根据标准荧光强度关系推算品钙离浓度
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