Product Specification:
Host: | Rabbit |
Isotype: | IgG |
Immunogen: | Synthetic peptide (CDYKDDDDK) coupled to KLH. |
Species reactivity: | Species independent |
Specificity: | Recognizes FLAG-tagged proteins. |
Applications: | ELISA, WB |
Recommended Dilutions/Conditions: | Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
Purity Detail: | Immunoaffinity purified. |
Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
Shipping: | Shipped on Blue Ice |
Long Term Storage: | -20°C |
Scientific Background: | FLAG-tag stands for an octapeptide with a sequence of DYKDDDDK. It is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It is widely used for affinity chromatography to purify recombinant proteins from variety of systems. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence, such as in ELISA, Western Blot, etc. |
Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. LLC |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
此贴是外泌体学习、相关答疑、战友互助、资源交换的专题帖,欢迎各位道友加入,互相交流学习,如有外泌体相关问题请留言,楼主会做解答。
版主yj1984ren留言:
欢迎!
小胶质细胞(microglia)是神经胶质细胞的一种,相当于脑和脊髓中的巨噬细胞,是中枢神经系统(CNS)中的第一道也是最主要的一道免疫防线。小胶质细胞大约占大脑中的神经胶质细胞的20%。小胶质细胞不停地清除着中枢神经系统中的损坏的神经,斑块及感染性物质。无数临床上和神经病理学研究表明激活的小胶质细胞在神经退化类疾病的发病机理中起到十分重要的作用,如帕金森病,多发性硬化和阿兹海默症等。但是过多激活或失控的小胶质细胞会引起神经毒性。他们是促炎因子和氧化应激的重要来源,如肿瘤坏死因子(TNF),一氧化氮,白介素等有神经毒性的物质
外泌体参与细胞间通讯,目前对外泌体的研究兴趣日益增长,小编整理一些外泌体相关的数据库,供研究者学习使用。
ExosomeDatabase
www.ExosomeData.com
EVpedia--Databaseofexosomalproteome,transcriptome,andlipidome
http://www.evpedia.info/
Apublicdatabaseforextracellularvesiclesresearch.
EVpediaisanintegratedandcomprehensiveproteome,transcriptome,andlipidomedatabaseofEVsderivedfromarchaea,bacteria,andeukarya,includinghuman.
EVpediaprovidesanarrayoftools,suchassearchandbrowsetoolsforvesicularproteins,comparisonofvesiculardatasetsbyorthologidentification,GeneOntologyenrichmentanalysesandnetworkanalysesofvesicularproteins.FurThermore,EVpediaprovidesdatabasesofvesicularmRNAs,miRNAs,andlipids.Thus,EVpediamightserveasausefulcommunityresourcetotriggertheadvancementofsystematicandcomprehensivestudiesofEVsandtounveilthefundamentalrolesofEVs.
ExoCarta--Databaseofexosomalproteins,RNAandlipids.
www.exocarta.org/
ExoCarta:databaseofexosomalproteins,RNAandlipids.
ExoCarta,anexosomedatabase,provideswiththecontentsthatwereidentifiedinexosomesinmultipleorganisms.
Vesiclepedia--Databaseofexosomalproteins,RNAandlipids
http://www.microvesicles.org/
Acommunitycompendiumforextracellularvesicles
Vesiclepedia,amanuallycuratedcompendiumofmoleculardata(lipid,RNAandprotein)identifiedindifferentclassesofEVs.Currently,Vesiclepediacomprises35,264protein,18,718mRNA,1,772miRNAand342lipidentriesencompassedfrom341independentstudiesthatwerepublishedoverthepastseveralyears.
ExosomeGeneOntologyAnnotationInitiative
http://www.ebi.ac.uk/GOA/exosome
Thisinitiative,undertakenbytheUniProtcuratorsattheEBI,involvesthemanualassignmentofGOannotationstohumanexosomalproteinsbycuratorsreADIngthescientificliteratureandassigningtotheproteinsGOtermsthatdescribetheirBIOLOGicalroles.CuratorsworkingonthisprojectarerequestingnewGOtermsspecifictoexosomeproteinbiologytofullycaptureliteraturefindings.
exoRBase--DatabaseofexosomalcircRNA、LncRNAandmRNA
http://www.exorbase.org/
exoRBaseisarepositoryofcircularRNA(circRNA),longnon-codingRNA(lncRNA)andmessengerRNA(mRNA)derivedfromRNA-seqdataanalysesofhumanbloodexosomes.Experimentalvalidationsfrompublishedliteraturearealsoincluded.
exoRBasefeaturestheintegrationandvisualizationofRNAexpressionprofilesbasedonnormalizedRNA-seqdataspanningbothnormalindividualsandpatientswithdifferentdiseases.
UrinaryExosomeProteinDatabase
https://hpcwebapps.cit.nih.gov/ESBL/Database/Exosome/
ThisdatabaseofurinaryexosomeproteinsisbasedonpublishedproteinmassspectrometrydatafromtheNHLBIEpithelialSystemsBiologyLaboratory(ESBL).Alldataarefromurinaryexosomesisolatedfromhealthyhumanvolunteers.
Thiscurrentdatabasecontainsproteinidentificationofhumanurinaryexosomesusingtwodifferentmassspectrometeranalyzers.
exRNAAtlas--DatabaseofexosomalRNA
http://exrna-atlas.org/
TheexRNAAtlasisthedatarepositoryoftheExtracellularRNACommunicationConsortium(ERCC),whichincludessmallRNAsequencingandRT-qPCR-derivedexRNAprofilesfromhumanandmousebiofluids.AllRNA-seqdatasetsareprocessedusingversion4oftheexceRptsmallRNA-seqpipelineandERCC-developedqualitymetricsareuniformlyappliedtothesedatasets.
editedbyAlexfromUmibio(Shanghai)Co.Ltd
外泌体提取试剂盒试用装,免费申请请邮件至umibio@umibio.cn
这个PPT是故障的,里面内容不全,大家不要下载,请大家和版主说让他把这篇帖子删除我重新再发
外泌体提取与鉴定.pptx(2895.91k)
写了外泌体RNA的中文综述,想投核心期刊,最好是容易接收,审稿费便宜的,求推荐一下,谢谢……
请教各位大神,大家知道怎样对外泌体表面蛋白进行定量吗?看有文献用Western,还有裂解外泌体后用BCA进行定量,请问裂解后的外泌体蛋白定量应该是外泌体全部蛋白的吧,应该不只是表面蛋白(CD63这种)吧,还有这个BCA是怎么操作呢?有没有很可靠地能表示外泌体表面蛋白的定量方法?另外,怎么根据外泌体表面蛋白的量来算出外泌体的量呢?还有外泌体文献报道的外泌体浓度为μg/ml,这种浓度是怎样测出来的呢?还有表示外泌体10^9/ml这个测外泌体个数的应该是通过NTA吧?求大神分享外泌体定量的方法?急急急~
exosome,外泌体,是由细胞分泌到外周体液进行细胞间以及细胞与远程组织、器官之间进行信息交流的一种媒介。当前逐渐成为国际研究热点。目前国内刚刚起步,虽然研究成果较国外研究机构尚有不少差距,但已有迎头赶上之势。
外泌体只是胞外囊泡的一种,目前尚未有明确定论胞外囊泡中其它成分的功能。
为了跟随时代科学研究进步的步伐,我建议开通“胞外囊泡及外泌体”讨论区,以促进该领域的充分讨论和合作,弥补当前该领域讨论内容分散,资料不容易汇集等缺点。同时也为广大胞外囊泡及外泌体医学研究者提供一个更好的交流空间。
各位前辈好,科研新手要研究尿外泌体,想了解一下研究尿外泌体比较大咖的相关国内外团队有哪些。今天就要汇报了。还请各位赏脸赐教一些。需要按照国内和国外列成两列。
暂无品牌问答