| Rotenoneinhibitor of the mitochondrial complex I electron transport chain |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
Nature.2018 Nov;563(7731):407-411.Nature.2018 Jun 13.Nature.2018 Jun 27.Nature.2018 Mar 29;555(7698):673-677.Nature.2017 Sep 7;549(7670):96-100.Nature.2016 Apr 21;532(7599):398-401.
Science.2016 Aug 5;353(6299)594-8Nat Nanotechnol.2017 Dec;12(12):1190-1198.Nature Biotechnology.2017 Jun;35(6):569-576Nat Med.2018 Sep 17.Cell.2018 Dec 21. pii: S0092-8674(18)31561-7.Cell.Available online 25 October 2018.Cell.2018 Sep 27. pii: S0092-8674(18)31183-8.Cell.2018 Jun 28;174(1):172-186.e21.Cell.2018 Feb 22;172(5):1007-1021.e17.Cell.2017 Nov 30;171(6):1284-1300.e21.Cell.2017 Aug 17. pii: S0092-8674(17)30869-3.Cell.2017 Jul 13;170(2):312-323Nat Med.2018 Jan 29.Nat Med.2017 Nov;23(11):1342-1351.Cell.2017 Apr 6;169(2):286-300.Cell.2015 Aug 27;162(5):987-1002.Cell.2015 Feb 12;160(4):729-44.Nature Medicine.2017 Apr;23(4):493-500.Cancer Cell.2018 May 14;33(5):905-921.e5.Cancer Cell.2018 Apr 9;33(4):752-769.e8.Cancer Cell.2018 Mar 12;33(3):401-416.e8.Cancer Cell.2017 Aug 14;32(2):253-267.e5.Nat Methods.2018 Jul;15(7):523-526.Cell Stem Cell.2018 May 3;22(5):769-778.e4.Cell Stem Cell.2017 Nov 20. pii: S1934-5909(17)30375-2.Quality Control & MSDS
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- Purity = 98.44%
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- HPLC
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)
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Chemical structure
| Cell experiment [1]: | |
Cell lines | differentiated SH-SY5Y neuroblastoma cells |
Preparation method | The solubility of this compound in DMSO is ≥77.6mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 50 nM; 21 days |
Applications | In differentiated SH-SY5Y neuroblastoma cells, Rotenone (50 nM) produced a biphasic survival curve with ~40% loss by 6 days and a second decline to ~60% loss between 18 and 21 days. Mitochondrial movement velocities were reduced at 8 days. |
| Animal experiment [2]: | |
Animal models | 20–25-weekold female BALB/c mice |
Dosage form | (rotenone: 0.35 mg/kg; DMSO: 9.86 mg/kg) or vehicle (DMSO: 9.86 mg/kg); injected into the right-side nasal cavity of mice; once a day for 2 weeks |
Application | In mice, rotenone attenuated the olfactory function and retarded the inhibitory input into the mitral cells, which are output neurons in the olfactory bulb (OB). Rotenone also caused neurite degeneration of DA neurons in the substantia nigra. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1].Borland MK, Trimmer PA, Rubinstein JD, et al. Chronic, low-dose rotenone reproduces Lewy neurites found in early stages of Parkinson"s disease, reduces mitochondrial movement and slowly kills differentiated SH-SY5Y neural cells. Mol Neurodegener, 2008, 3: 21. [2]. Sasajima H1, Miyazono S, Noguchi T, et al. Intranasal Administration of Rotenone to Mice Induces Dopaminergic Neurite Degeneration of Dopaminergic Neurons in the Substantia Nigra. Biol Pharm Bull. 2017;40(1):108-112. | |
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| Cas No. | 83-79-4 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | (2R,6aR,12aS)-8,9-dimethoxy-2-(prop-1-en-2-yl)-1,2,12,12a-tetrahydrochromeno[3,4-b]furo[2,3-h]chromen-6(6aH)-one | ||
| Canonical SMILES | O=C1[C@H](C2=CC(OC)=C3OC)[C@@H](COC2=C3)OC4=C1C=CC5=C4C[C@H](C(C)=C)O5 | ||
| Formula | C23H22O6 | M.Wt | 394.42 |
| Solubility | ≥77.6mg/mL in DMSO | Storage | Store at RT |
| Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
Rotenone is an inhibitor of the mitochondrial complex I electron transport chain with IC50 value of 1.7 - 2.2 μM.
Electron transport chain (ETC) is a series of compounds that transfer electrons from electron donors to electron acceptors and transfer protons (H+ ions) across a membrane. The proton gradient drives ATP synthesis. Complex I is one of the main sites of production of superoxide.
Rotenone is an inhibitor of the mitochondrial complex I electron transport chain. In the transformed cell line HEK 293 and cancer cell lines U87, rotenone (50 μM) induced cell death by 30% and 40% respectively in a dose dependent way, which was mediated by reactive oxygen species (ROS). Also, rotenone significantly induced autophagy formation [1]. In SH-SY5Y cells, rotenone induced cell apoptosis in a caspase-dependent way. Also, rotenone induced phosphorylation of p38 MAP kinase, c-Jun and JNK, which indicated activation of the p38 and JNK pathways [2]. In differentiated SH-SY5Y neuroblastoma cells, rotenone (50 nM) induced cell death by 60% and slowed mitochondrial movement. While rotenone didn’t induce the formation of resembling Lewy bodies [3].
References:[1]. Chen Y, McMillan-Ward E, Kong J, et al. Mitochondrial electron-transport-chain inhibitors of complexes I and II induce autophagic cell death mediated by reactive oxygen species. J Cell Sci, 2007, 120(Pt 23): 4155-4166.[2]. Newhouse K, Hsuan SL, Chang SH, et al. Rotenone-induced apoptosis is mediated by p38 and JNK MAP kinases in human dopaminergic SH-SY5Y cells. Toxicol Sci, 2004, 79(1): 137-146.[3]. Borland MK, Trimmer PA, Rubinstein JD, et al. Chronic, low-dose rotenone reproduces Lewy neurites found in early stages of Parkinson"s disease, reduces mitochondrial movement and slowly kills differentiated SH-SY5Y neural cells. Mol Neurodegener, 2008, 3: 21.
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求助各位大神，点击Downloadfulltable无法下载，总是出现错误，已经排除网络的问题，不知道是否还有其他方法下载，上面有一个Datatable的表格，不知道是不是芯片平台文件？
做蛋白质和microRNA的关系，那么先找出差异的蛋白质还是找出差异的microRNA好呢？有没有做ITRAQ和MICRORNA芯片好的公司推荐，非常感谢！
中唐临界风险，33岁，第一胎，然后羊水穿刺加CMA基因芯片检查，染色体核型正常，4维彩超正常，医生说发育速度偏慢，但还算正常范围，CMA结果异常，如照片
1、一张芯片上可以同时分析成百上千的探针，这样的情况可以检测多少位点？
2、基因芯片技术通量会有多大？怎么计算？
研究蛋白质芯片的意义
1。蛋白质是基因表达的最终产物,接近生命活动的物质层面；
2。探针蛋白特异性高、亲和力强,可简化样品前处理，甚至可直接利用生物材料(血样、尿样、细胞及组织等)进行检测；
3。适合高通量筛选与靶蛋白作用的化合物；
4。有助于了解药物或毒物与其效应相关蛋白质的相互作用。
蛋白质芯片的分类：
1.蛋白质检测芯片
2.蛋白质功能芯片
蛋白质芯片的制备：
1。固相载体及其处理
载体（滴定板、滤膜、凝胶、载玻片）
2。蛋白质的预处理
选择具有较高纯度和完好生物活性的蛋白进行溶解
3。点制微阵列
可使用点制基因微阵列的商品化点样仪或喷墨法等
4。膜为载体：芯片放入湿盒，37°C1h
载玻片为载体：化学修饰产生醛基固定蛋白
5。微阵列的封闭固定微阵列上的蛋白样点
主要封闭试剂：BSA或Gly
相关链接:
全部有关生物芯片的实验方法技术(protocol)
生物芯片相关仪器及芯片・芯片扫描仪・芯片点样仪・生物芯片・生物芯片系统・其它
生物芯片技术服务
核酸分析类试剂
AFLP分析|SNP基因分型|线粒体DNA基因分型|其它基因分型|DNA指纹试剂盒|DNA测序试剂|核酸电泳凝胶|核酸标准品|凝胶纯化试剂盒|核酸染色|转座工具|其它
从GEO和ARRAYEXPRESS下载了miRNA的表达数据矩阵，但是结果不太一样，大致两种：①数值从个位数到几万不等，而且没有小数点；②数值在10上下，有小数点。见图
请问怎么知道数据是否经过了log转换？有注释文件说明吗？还是直接判断①是没转换的，②是转换的？
无创dna检测8号染色体缺失32.99mb，羊穿正常，基因芯片检测还是8号染色体杂合性缺失32.2mb，请教专家孩子能要吗？万分感谢
请问，我通过基因芯片筛选差异基因，有上调的也有下调的，即FC有正值，亦有负值，那么负值怎么取log？谢谢
暂无品牌问答
