液态芯片原理编码微球:分别用不同配比的两种荧光染料将直径5.6μm的聚苯乙烯微球(Beads)染成不同的荧光色,从而获得多达100种经荧光编码的微球。交联探针、抗体或抗原:把针对不同检测物的核酸探针、抗体或抗原以共价方式结合到特定荧光编码的微球上。检测反应:先把针对不同检测物的、用不同荧光色编码的微球混合,再加入被检测物(可以是血清中的抗原、抗体或酶等,也可以是PCR产物)。悬液中的微球与被检测物特异性结合,结合物被标记上荧光物质。激光分析:微球成单列通过两束激光,一束判定微球的荧光编码;另一束测定微球上的报告分子的荧光强度。 1)液态芯片目前仍然处于基础研究方面,虽然已经有部分临床研究,但可能离大规模应用还有一段距离。2)常见的应用如肿瘤、内分泌、自身免疫等的检测,也有人将其用于细胞因子谱判断。3)下面是一些液态芯片相关经典的文献,有时间参考一下1.HanyEzzeldin,YoshihiroOkamoto,MartinR.Johnson,etal.AHigh-qhroughputDenaturingHigh-PerformanceLiquidChromatographyMethodfortheIdentificationofVariantAllelesAssociatedwithDihydropyrimidineDehydrogenaseDeficiency.AnalyticalBiochemistry,2002,306:63-73..2.CarsonRT,VignaliDA.Simultaneousquantitationof15cytokinesusingamultiplexedflowcytometricassay.JImmunolMethods,1999,30.227(1-2):41-52..3.Karlar,G.A,M.Jeddi-Tehrani,F.Shokri.DiminishedThlandTh2cytokineproductioninhealthyadultnonresponderstorecombinanthepatitisBvaccine.Scand.JImmunol,2002,55:311-314..4.TaylorJD,BrileyD,NguyenQ,etal.Flowcytometricplatformforhighthroughputsinglenucleotidepolymorphismanalysis.Biotechniques,2001.30(3),661-666.668-669..5.WilcodeJager,HenkteVelthuis,BerentJ.Prakken,etal.SimultaneousDetectionof15HumanCytokinesinaSingleSampleofStimulatedPeripheralBloodMononuclearCeils.ClinicalandDiagnosticLaboratoryImmunology,2003,10(1):133-139..6.lannoneMA.Microsphere-htsedmolecularcytometry.ClinLabMed,2001.21(4):731-742..7.JoosTO,StollD,TempiinMF.Miniaturisedmultiplexedimmunoassays.CurrOpinChemBiol,2002,6(1):76-80..8.DunbarSA,VanderzeeCA,OliverKG,etal.Quantitative,multiplexeddetectionofbacterialpathogens:DNAandproteinapplicationsoftheLuminexLabMAPsystem.JMicrobiolMethods,2003,53(2):245-252..9.DavidOpalka,CharlesE.Lachman,StefaniA.MacMullen,etal.SimuhaneousQuantitationofAntibodiestoNeutralizingEpitopesonVirus-LikeParticlesforHumanPapillomavirusTypes6,11,16,and18byaMultiplexedLuminexAssay.ClinicalandDiagnosticLaboratoryImmunology,2003,10(1):108-115.. 相关链接: