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GenWay/Mouse IL-28 ELISA Kit/GWB-SKR187/96 Tests
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4000-520-616
Description:
ShortDescription:ThisproductisaSandwichELISAwithadetectionmethodofColorimetric450nm.TheMurineIL-28ELISAKitallowsforthedetectionandquantificationofendogenouslevelsofnaturaland/orrecombinantMurineIL-28proteinswithintherangeof32-4000pg/ml.TheMurineIL-28ELISAiscapableofrecognizingbothrecombinantandnaturallyproducedMurineIL-28proteins.Theantigenslistedbelowweretestedat100ng/mlanddidnotexhibitsignificantcrossreactivityorinterference.•HumanIFN-alphaA/D,IFN-alphaA,IFN-alphaB2,IFN-alphaG,IFN-alphaI,IFN-beta1aAdditionalInformation:
Name | MouseIL-28ELISAKit | ||||||||||||||||||||||
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RelatedProductNames | Interleukin-28A/B,InterferonlamBDa-2,3 | ||||||||||||||||||||||
GeneSymbol | IFNL2 | ||||||||||||||||||||||
GeneFullname | interferonlambda2 | ||||||||||||||||||||||
Geneid | 330496;338374 | ||||||||||||||||||||||
AliasSymbols | EG330496,Ifnl2,IFN-lambda-2,IL-28A,Interferonlambda-2,Interleukin-28A | ||||||||||||||||||||||
ProteinName | Interferonlambda-2 | ||||||||||||||||||||||
DescriptionofTarget | Cytokinewithantiviral,antitumourandimmunomodulatoryactivities.Playsacriticalroleintheantiviralhostdefense,predominantlyintheepithelialtissues.ActsasaligandfortheheterodimericclassIIcytokinereceptorcomposedofIL10RBandIFN | ||||||||||||||||||||||
SwissprotID | Q4VK74 | ||||||||||||||||||||||
ProteinAccessionNum | NP_001019844.2 | ||||||||||||||||||||||
NucleotideAccessionNum | NM_001024673.2 | ||||||||||||||||||||||
SpeciesReactivity | Mouse | ||||||||||||||||||||||
AssayInfo | QuantitativeColorimentricSandwichELISA | ||||||||||||||||||||||
Sensitivity | 32pg/mL | ||||||||||||||||||||||
KitRange | 32-4,000pg/mL | ||||||||||||||||||||||
StandardRange | 32-4000pg/ml | ||||||||||||||||||||||
SampleType | Celllysates,seraandplasma | ||||||||||||||||||||||
ProductFormat | 1x96-WellPlateor12x8-WellStrips | ||||||||||||||||||||||
Specimen/Volumes | RefertoManual | ||||||||||||||||||||||
SwissProtNumber | Q4VK74/Q8CGK6 | ||||||||||||||||||||||
Specificity | TheMurineIL-28ELISAiscapableofrecognizingbothrecombinantandnaturallyproducedMurineIL-28proteins.Theantigenslistedbelowweretestedat100ng/mlanddidnotexhibitsignificantcrossreactivityorinterference. •Human:IFN-αA/D,IFN-αA,IFN-αB2,IFN-αG,IFN-αI,IFN-β1a | ||||||||||||||||||||||
Applications | ELISA | ||||||||||||||||||||||
GINumber | 330496/338374 | ||||||||||||||||||||||
Format | ELISAKit.Refertomanualforspecificcomponentformats | ||||||||||||||||||||||
StABIlity | 6months | ||||||||||||||||||||||
ReconstitutionandStorage | 4C/6Months | ||||||||||||||||||||||
Reactivity | Mouse | ||||||||||||||||||||||
KitSize | 12x8-WellMicrostrips | ||||||||||||||||||||||
KitPrinciple | TheAvivaMurineIL-28ELISAKitcontainsthecomponentsnecessaryforquantitativedeterminationofnaturalorrecombinantmIL-28concentrationswithinanyexperimentalsampleincludingcelllysates,serumandplasma.Thisparticularimmunoassayutilizesthequantitativetechniqueofa"Sandwich"Enzyme-LinkedImmunosorbentAssay(ELISA)wherethetargetprotein(antigen)isboundina"sandwich"formatbytheprimarycaptureantibodiescoatedtoeachwell-bottomandthesecondarydetectionantibodiesaddedsubsequentlybytheinvestigator.ThecaptureantibodiescoatedtothebottomofeachwellarespecificforaparticularepitopeontheMurineIL-28cytokinewhiletheuser-addeddetectionantibodiesbindtoepitopesonthecapturedtargetprotein.Amideachstepoftheprocedure,aseriesofwashstepsmustbeperformedtoensuretheeliminationofnon-specificbindingbetweenproteinstootherproteinsortothesolidphase.Afterincubationand"sandwiching"ofthetargetantigen,aperoxidaseenzymeisconjugatedtotheconstantheavychainofthesecondaryantibody(eithercovalentlyorviaAvidin/Streptavidin-Biotininteractions),allowingforacolorimetricreactiontoensueuponsubstrateaddition.WhenthesubstrateTMB(3,3’,5,5’-Tetramethylbenzidine)isadded,thereactioncatalyzedbyperoxidaseyieldsabluecolorthatisrepresentativeoftheantigenconcentration.Uponsufficientcolordevelopment,thereactioncanbeterminatedthroughadditionofStopSolution(2NSulfuricAcid)wherethecolorofthesolutionwillturnyellow.Theabsorbanceofeachwellcanthenbereadbyaspectrophotometer,allowingforgenerationofastandardcurveandsubsequentdeterminationofproteinconcentration. | ||||||||||||||||||||||
KitComponent |
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Storage | 4°C/6Months | ||||||||||||||||||||||
LeadTime | Domestic:within1weekdelivery International:1week | ||||||||||||||||||||||
IntendedUse | ResearchUseOnly |