- Description
- Additional
Description
Details
Description:Mouse monoclonal antibody (mAb) to H. pylori cytotoxin associated gene A (CagA) protein
Purification:Protein G affinity purified
Product Type:Primary antibody
Target Protein:H. pylori cytotoxin associated gene A protein
Immunogen:A 32KD recombinant protein cloned from the highly immunogenic region of H. pylori cytotoxin associated gene A.
Fusion Myeloma:Sp2/0-Ag14
Specificity:The mAb reacts with H. pylori CagA.
Cross-Reactivity:Cross-reaction with other proteins has not been found.
Host / Isotype:Mouse, IgG1 Kappa
Formulation:Lyophilizedfrom a solution in 0.01M PBS, pH 7.0
Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Storage: Store at -20oC
Research Area:Bacteriology
Background:
Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.
Application:
ELISA:Clone 5H10 can be used as capture antibody in sandwich ELISA to detect the recombinant CagA antigen in combination with HRP conjugated anti-CagA clone 5C6, clone 3C10 and clone 3C1. In addition, clone 5H10 coated wells selectively react with cell lysate of CagA containing H. pylori strain when HRP conjugated anti-CagA clone 5C6 is used as detection antibody.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Additional
Additional Information
Product Specificity | mAb anti-HP CagA, 5H10 |
---|---|
Application | EIA |
Size | 0.1 mg |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
试剂里面含有的是病毒的抗原,现在基本都是人工合成或提取的,跟病毒完全是两回事
病毒液通过pre-filterdisc的时候是不是要制造一个真空环境产生压力差使液体通过?这个时候是用自备的针管还是试剂盒配的5毫升的针管啊?
如果PCR产物不是很纯,或者PCR扩增条带比较小,PCR产物前面又有较多引物二聚体时,用胶回收,其余用PCR产物纯化试剂盒。
pcr纯化试剂盒和胶回收试剂盒的区别:
PCR纯化试剂盒:是直接水溶解的PAC产物就可以回收,回收效率高,但是只适合单一条带需要纯化测序的时候使用。
PCR凝胶试剂盒:是在PCR产物是混合物,有多条杂带的情况下,先跑胶将杂带分离,然后在将所要的条带位置的胶切下回收,后者的回收效率低,但是很纯净。
胶回收试剂盒操作步骤:
配制琼脂糖EB凝胶,电泳以分离DNA片段。任何类型或等级的琼脂糖都可以使用。
电泳足够时间后,在紫外灯下小心地把所需的DNA的片段切下来。并尽量去除多余的凝胶。
称取空离心管的重量,切下带目的片段的凝胶装在1.5ml离心管中并称其重量,求出凝胶块的重量,近似地确定其体积。一般情况下,凝胶的密度为1g/ml,于是凝胶的体积与重量的关系可按下面换算:凝胶薄片的重量为0.2g 则其体积为0.2ml;加入等倍凝胶体积的Binding Buffer,把混合物置于55℃~65℃水浴中温浴7min至凝胶完全融化,其间每隔2-3分钟混匀一次;
转移700μl的DNA-琼脂糖溶液到一个HiBindTM DNA柱子,并把柱子装在一个干净的2ml收集管内,室温下,10,000×g离心1min,弃去液体。
将柱子重新套回收集管中,加300μl Binding Buffer至HiBind DNA 柱子中;室温下,10,000×g离心 1分钟,去弃滤出液;这一步相当关键,不要忽略此步。
将柱子重新套回收集管中,加入700μl SPW Wash buffer至HiBind DNA柱子中,室温下,10,000×g离心1分钟,去弃滤出液;注:SPW Wash buffer在使用前必须按瓶子标鉴要求用无水乙醇进行稀释。
将柱子重新套回收集管中,重复加入700μl SPW Wash buffer至HiBind DNA柱子中,室温下,10,000×g离心1分钟,去弃滤出液;
弃去液体,将空柱子重新套回收集管中,10,000×g离心1min以甩干柱基质残余的液体。
这步可以去除柱子基质上残余的乙醇,不要省略此步―――对得到好的DNA产量是十分重要的。
把柱子装在一个干净的1.5ml离心管上,加入30~50μl洗脱液或灭菌水上柱子膜上,10,000×g离心1分钟,离心管中的溶液就是纯化的DNA产物,保存于-20度。
暂无品牌问答