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IBL/Cortisol Saliva ELISA/RE52611/

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Kitsize12x8MethodELISAIncubationtime1x2h,1x30minStandardrange0.15-30ng/mLSpecimen/Volumes50µLsalivaSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: CortisolSalivaELISASensitiveandreliableThenewIBLInternationalCortisolSalivaELISAkitisanexcellentalternativetothecurrentcomplexandrelativelyexpensiveLC-MS/MSmethodtodeterminecortisolinsalivaasaccuratelyaspossible.AdvantagesofthenewIBLCortisolSalivaELISACalibrationtotheLC-MS/MSreferencemethod; IBL-ELISA=1.015xLCMS-0.028,R=0.99Asfewas6standardsand2controls;Simpleprocessing;Clearlydefinedreferencevalues.DiurnalrhythmThenormalcortisollevelsinhumansalivadependonthetimeofday.Thetimingofthecortisolpeak,however,doesnotdependontheactualtimeofdayordaylight,butcorrelateswiththeusualwake-uptime.Thenewreferencerangeshavebeencarefullydeterminedonthebasisof100dayprofiles.Startingwithbatchno.ECO137,thefollowingreferencevalueshavetobeconsidered:Timeafterawakening(h)Cortisol(saliva)Range-RE52611(♂/♀;>6y;n=100;5%-95%percentile)Median Range(µ/dL)MedianRange(nmol/L)(µ/dL) 5% 95%(nmol/L)5%95%Awakening0.3430.1130.8039.473.1222.170.50.4780.2001.07613.195.5229.7010.3840.1010.93610.602.7925.8220.2340.0830.5746.442.2915.8550.1500.0740.3554.142.049.7980.1160.0550.3143.201.538.67120.0820.0320.3222.260.878.89SensitiveandreliableTheinvestigationofcortisolinsalivaisindicatedinthediagnosticwork-upofclinicalissuesinvariousscientific/medicalfields,particularlyendocrinology,psychology,sportsmedicine,pediatrics,anti-agingmedicineandveterinarymedicineMeasuringthefreecortisollevelsinsalivaisaverysimpleandconvenientwaytocheckthefunctionoftheadrenalgland.Persistentlyelevatedordecreasedcortisollevelsareoftenfoundinpatientswithadrenaldysfunction.Adrenaldysfunctioncanoccurinmanyotherdiseases,suchasfibromyalgia,hypothyroidism,chronicfatiguesyndromeandprematuremenopause.Thesalivarycortisoltestcanalsobeusedinpatientswithconditionssuchasreducedstresstolerance,fatigue,sleepdisordersanddepression.StandardizedreagentsOurSalivaELISAproductlinehasbeenrevisedsothatthemainsteroids(cortisol,testosterone,progesterone,DHEA)maybeprocessedinasimilarway:Thesubstrate,washingandstopsolutionsareinterchangeable;Theenzymeconjugate,thesubstrateandstopsolutionsareuniformlyPipettedwith100µL;Thekitscontainasfewas6standardsand2controls;Only50µLsamplevolumearerequired;Allkitsusethesamesamplediluentbuffer.Thankstothesimilarprocessingandassembling,theassayscaneasilybeperformedincombination.ExcerptfromtheInstructionsforUseEnzymeimmunoassayforthein-vitrodiagnosticquantitativedeterminationoffreeCortisolinhumansalivaandoftotalCortisolindilutedserumasanaidintheassessmentofCushingSyndromeandAddison’sDisease.Cortisolisacorticosteroidorglucocorticoidproducedbytheadrenalcortex.Itisusuallyreferredtoasthe"stresshormone"asitisinvolvedinresponsetostressandanxiety.Itincreasesbloodpressureandbloodsugar,andreducesimmuneresponses.FreecortisolmonitoringisindicatedindiseaseswithabnormalglucocorticoidproductionsuchasCushing`ssyndromeandAddison’sdisease.Becauseofthediurnalfluctuationofcortisollevels,itisnecessarytotakeseveralsamplesforanindividualcortisolprofileorduringdynamictestslikethedexamethasone-suppressionorACTHstimulation(Westerman,Clin.Lab.(2004)50;11-24).FurThermoreadrenaldysfunctioncanbepointedoutthroughconcomitantmeasurementofthemostimportantstresshormonescortisolandDHEA.Cortisolhelpsevaluatethestressresponse,whereasDHEAhelpsdeterminethestressadaptation.Adrenalimbalancemaybeafactorinmanyrelatedclinicalconditionsthatincludee.g.fibromyalgia,hypothyroidism,chronicfatiguesyndromeanddepression.OurCortisolsalivaisverywellpublished,pleasedonothesitatetocontactusforfurtherinformation.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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