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IBL/Dehydroepiandrosterone (DHEA) Saliva ELISA/RE52651/

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RE52651

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Kitsize12x8MethodELISAIncubationtime1x2h,1x30minStandardrange20-2160pg/mlSpecimen/Volumes50µLsalivaSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: Dehydroepiandrosterone(DHEA)SalivaELISAThenewIBLInternationalDHEASalivaELISAcorrelatesverywelltotheLC-MS/MSreferencemethod,andistheonlycommerciallyavailableassaywithclearspecifiedreferencevalues.ThenormalDHEAconcentrationalterssignificantlywithage,genderandtimeoftheday.Duringtheday,DHEAvalueshavethehighestlevelinthemorninganddecreaseoverthedaytothelowestlevelduringnighttime.ThispatternissomewhatsimilartoCortisol.Forthisreasonclearindicationaboutthereferencevaluesisofutmostimportance.AdvantagesoftheimprovedIBLDHEASalivaELISACalibrationtotheLC-MS/MSreferencemethod y=0.91x–13.35;r=0.96;N=130Measuringrangeupto2,160pg/mLClearlydefinedreferencevaluesTheTMB,stopsolutionandwashsolutionareinterchangeablewithinCortisol,TestosteroneandProgesteronesalivaELISA(RE52611,RE52631andRE52281)Thenewreferencerangeshavebeencarefullydeterminedonthebasisof140dayprofiles,withrespecttothegender,ageandtimeoftheday.Startingwithbatchno.EDE113,thefollowingreferencevalueshavetobeconsidered: MorningEvening Agen5%-95%percentileMedian5%-95%percentileMedian[pg/mL][pg/mL][pg/mL][pg/mL]Females14-3939101.0-776.8277.128.1-414.5129.740-853153.2-418.3240.935.4-241.291.714-85(total)7068.0-676.0251.935.4-396.8110.3Males16-3945104.5-790.1362.974.2-487.9161.740-762538.1-501.6264.024.7-622.1107.016-76(total)7059.2-745.1326.551.1-482.0141.4Itisrecommendedthateachlaboratoryestablishesitsownrangeofnormalvalues. NumerousApplicationfieldsDuetotheubiquitousoccurrenceofDHEA,itsdeterminationisusefulinmanyfieldsofmedicaldiagnosticsandinresearch,notonlyinendocrinologyandanti-agingmedicinebutalsoinneuroendocrinologyandveterinarymedicine.DHEAisastressMarkerinpsychologyandinsportmedicine.PulsatiledynamicsDuetothepulsatiledynamicsofDHEAsecretionrepeatedsalivasamplingisrecommended.Werecommendcollecting3to5salivasampleswithin2hours.Inthelaboratory,equalvolumesoftheindividualsalivasamplescanbemixed.ThismixedsampleresultsinameanfreeDHEAvalue,whichrepresentstheactivehormoneconcentrationinareproducibleway.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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