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IBL/IGF-I ELISA/MD58011/

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MD58011

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4000-520-616

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Kitsize12x8MethodELISAIncubationtime1x1h(RTS),1x30min(RTS),1x15minStandardrange42-1050ng/mL(1:21dil.)or20-500ng/mL(1:10dil.)Specimen/Volumes10µLSerumorPlasmaSubstrate/isotopeTMB,450nmRegulatoryStatus:EU:CEDetailsfor: IGF-IELISAAnon-extractionassaywithhighspecificityThemeasurementofinsulin-likegrowthfactor-I(IGF-I)inserumisofespeciallyhighutilityinthediagnosticwork-upofgrowthdisturbances,particularlywithregardtohumangrowthhormone(hGH)deficiencyoracromegaly.ThemostimportantadvantageofmeasuringIGF-IoverhGHareitsstablecircADIanlevels,i.e.evenasinglemeasurementisofconclusivesignificance.Inviewoftheabove,measurementofIGF-Ishouldbeputonthefrontburnerinlaboratorydiagnosticwork-up.TheadvantagesofourELISAsare:non-extractionassay;highspecificityforIGF-I;nointerferencefromIGF-bindingproteins(IGFBP);onlyasamplevolumeof10µLrequired;onesampledilutionsuffices(usually1:21);calibratedagainsttheinternationalstandardofWHONIBSC02/254;veryhighsensitivity(0.09ng/mL);outstandingaccuracy--98.7%recovery;remarkableprecision:inter-assayvarianceof6.8%andintra-assayvarianceof6.7%.IBLInternational´sIGF-Ienzymelinkedimmunosorbentassay(ELISA)byIBLInternationalisusedformeasuringIGF-Iinhumanserum/plasma,cerebrospinalfluidandotherbodyfluidsaswellasinconditionedcellculturemediaofmanyhumancelllines.Whatismore,theassaycanalsobeusedforthemeasurementofIGF-Iinothermammalssuchasprimates,cattle,pig,sheep,horse,donkey,goat,dog,cat,rabbitandguineapig.However,thekitisnotsuitedforrat,mouseandchickensamplesInsulin-likegrowthfactorsIandII(IGF-I,IGF-II)playakeyroleinproliferation,differentiationandspecificfunctionsofmanycelltypes.IGF-IisidenticaltosomatomedinC(Sm-C)andisregulatedbythegrowthhormone(hGH/GH)andnutrition.Unlikemanyotherpeptidehormones,IGFsareboundtospecificbindingproteins(IGFBP)withahighaffinity.AsubstantialprobleminthemeasurementofIGF-IlevelsistheinterferencebyIGFBPs.Whenmeasuredinuntreatedserumsamples,inaccurateresultsareobtainedduetotheextremelyslowdissociationoftheIGF-I/IGFBP-3complexduringassayincubation.Forthisreason,onlyafractionofIGF-Iisavailableformeasurement.InterferencesaredeterminedbytheIGF-ItoIGFBPratiointhesample.Figure1:InterferencebyIGFBPinIGF-Imeasurements.KnownIGF-Iconcentrationsweremeasuredinthepresenceof0.5ng(left)and5ng(right)ofhIGFBP-3usingacommercialassay(□)andourIGFBP-blockedIBLassay(ӿ).Inordertocircumventthisproblem,asimpleassaywasdevelopedinwhichsamplesareacidifiedanddilutedusingabufferofaspecialcompositionpriortoperformingELISA.ClinicalSignificanceApartfromhGH,anumberofotherfactorsaffectserumIGF-Ilevels.Lowvaluesarefoundinmalnutrition/malabsorption,hypothyroidism,liverdisease,untreateddiabetesmellitus,chronicinflammatorydiseases,malignanciesandmultipletrauma.Elevatedlevels,ontheotherhand,arefoundinprecociouspubertyandobesity.InordertobeabletocorrectlyinterpretIGFmeasurements,itisofutmostimportancetoconsidertheage-dependentpatternofIGF-1levels,whichyoucanfindintheInstructionforUse.Table:NormalserumIGF-Ilevels(ng/mL)invariousTannerpubertalstages.Onlychildrenandadolescentsaged7-17wereincluded.PercentilesPubertätsstadium0.1th5th50th95thPubertalStage16110518633012851562985682311319635263134171268431693451652634317065Figure2:Age-dependentreferencerangeforIGF-Ilevelsingirls(ageinyears)Figure3:Age-dependentreferencerangeforIGF-Ilevelsinboys(ageinyears)Figure4:Age-dependentreferencerangeforserumIGF-Ilevelsinadults(ageinyears)Figure5:IGF-Ilevelsinhealthychildrenandadolescents(aged7.17)invariouspubertalstages(pooleddatafrombothgenders)ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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