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IBL/IGFBP-3 ELISA/MD58031/

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MD58031

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Kitsize12x8MethodELISAIncubationtime2x1h,1x30minStandardrange0.03-15150µg/LSpecimen/Volumes10µlSerum,PlasmaSubstrate/isotopeTMB,450nmRegulatoryStatus:EU:CEDetailsfor: IGFBP-3ELISAEnzymeimmunoassayformeasurementofIGFBP-3inhumanserumandheparinplasmafordiagnosticandscientificpurposes.ThisELISAuseshighlyspecificantibodies.NocrossreactivitytoothercomponentsoftheIGFsystemareknownandsmallsamplerequirementisidealforuseinpediatrics.AdvantagesofourIBLInternationaltotalIGFBP-3ELISA:-Detectionlimitof0.1ng/mL-Singlestandards:0.4,2,6,15,30ng/mL-Assay-range0.3–15150ng/mL-Intra-Assayvariance4.51%-Inter-Assayvariance6.3%-Broadlinearrange(1:20to1:1280)-2internalcontrols-Fast–incubationtimeof2.5hInsulin-likegrowthfactors(IGF)-Iand-IIareboundtospecificbindingproteins(IGFBPs)inthecirculation.Todaytheexistenceofatleastsixbindingproteinsandseveralrelatedproteinshasbeenshown.ThepredominatingIGFBPinbloodisIGFBP-3whichlargelydeterminesthetotalIGF-IandIGF-IIconcentration.PhysiologicalfunctionofIGFBPsismainlytoregulationofbioavailABIlityofIGF-Iand–II.IGFBP-3isthemostabundantIGFBPinhumanserum.ItbindsIGF-Iwithhighaffinityandisabletoformaternarycomplexwithacid-labilesubunit(ALS).IGFboundinthiscomplexisBIOLOGicalinactive.ActivationoccursbyproteasescleavingALSandIGFBP-3specifically.ClinicalRelevanceForclinicalpractice,themostimportantregulatoryfactorofIGFBP-3isGH.IGFBP-3measurementscorrelatesignificantlywithGHsecretion.SlowresponsetoGHandconstantcircADIanlevelsduringchronicdailyapplicationofGHsuggestthatIGFBP-3reflectstheGHsecretorystateoverdays.DeterminationofhumanIGFBP-3isdonefordiagnosticofGrowthHormonedeficiency.SerumIGFBP-3measurementsarealsosuitedforevaluatingthepotentialofapatienttorespondtoGHandforGHtherapymonitoring.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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