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IBL/Testosterone Saliva ELISA/RE52631/
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Kitsize12x8MethodELISAIncubationtime1x2h,1x30minStandardrange10-900pg/mLSpecimen/Volumes50µLsaliva/withoutextractionSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: TestosteroneSalivaELISAImprovedSalivaTestingofhormonelevelsEnzymeimmunoassayforthequantitativedeterminationoffreetestosteroneinhumansaliva.Themeasurementoftestosteroneisusedinthediagnosisandtreatmentofdisordersinvolvingthemalesexhormones(androgens),includingprimaryandsecondaryhypogonADIsm,andadditionally:withimpotenceinmales,infemalehirsutism(excessivehair),virilization(masculinization)duetotumors,polycysticovariesandadrenogenitalsyndromes.TheimprovedIBLInternationalTestosteroneSalivaELISAiscalibratedtotheLC-MS/MSreferencemethodandincludesinterchangeablereagentswithintheIBLsalivaELISAproductline.TheTestosteroneSalivaELISAcaneasilybeautomatedonopenplatformsliketheEVOlyzer®fromTecan.BenefitsoftheTestosteroneSalivaELISACalibrationtotheLC-MS/MSSimplifiedpipettingprotocolOptimizationforautomationAlignmenttoentireSalivalineHighSensitivityELISAvs.MassSpectronomyMassspectrometry(MS)isconsideredasthereferencemethodforsteroidhormonequantification.Thismethodologydoeshoweverrepresentalargeinvestmentandassuchisnotavailabletoalllaboratories.AlltheIBLsalivaassayshavebeendevelopedtocorrelatetothisreferencemethod,andevenmoreimportantly,onlyrequirelowsamplevolumeforquantificationofthesteroidhormones.Figure:ExcellentcorrelationofIBLInternationalTestosteroneSalivaELISAtotheLC-MS/MSreferencemethodConsistentandreliableprocessingwithinSalivaproductlineOurSalivaELISAproductline(Cortisol,Testosterone,Progesterone,DHEA)canbeprocessedinasimilarwayandbeeasilyperformedincombination:TheTMB,washingandstopsolutionsareinterchangeable;Theenzymeconjugate,thesubstrateandstopsolutionsareuniformlyPipettedwith100µL;Thekitscontainasfewas6standardsand2controls;Only50µLsamplevolumearerequired;Allkitsusethesamesamplediluentbuffer.AutomationTheIBLInternationalTestosteroneSalivaELISA(RE52631)canbeadaptedondifferentELISAprocessors.OpenautomatedplatformsliketheEVOlyzer®fromTecanhavebeentestedandshowgoodcorrelationtothemanualruns:Thecombineduseofassays,processscriptandautomatehastobevalidatedindividuallyonsitebyeachlaboratory.ApplicationsofFreeTestosteronedeterminationTestosteroneisasteroidhormone,producedinthetestesofmalesandinsmallerquantitiesintheovariesofwomen.InbothmenandwomenasubstantialamountisproducedbytheconversionofcirculatingDHEA-S,DHEAandandrostenedione.Itshowsadiurnalrhythm,withhighestlevelsinthemorningandanadiraroundmidnight.Testosteroneplaysanimportantroleinthedevelopmentofmalereproductivetissues,supportsincreasedmuscle,bonemass,andhairgrowthandisessentialforhealth,stamina,sexualfunction,cardiovascularhealth,andahealthyimmunesystem.Themeasurementoftestosteroneisusedinthediagnosisandthetreatmentofdisordersinvolvingandrogens,themalesexhormones.Theseincludeprimaryandsecondaryhypogonadism,disordersoriginatingfromtesticularproblemsordefectsofthehypothalamusorpituitaryglands.FurThermorewithimpotenceinmales,infemalehirsutism(excessivehair),virilization(masculinization)duetotumors,polycysticovariesandadrenogenitalsyndroms.TestosteronetogetherwithCortisollevelsalsorepresentsausefulparameterinstressresearchandsportsmedicine.MeasurementofTestosteroneinsalivaOnly1-2%ofthehormoneTestosteroneisunboundfromplasmaproteinsandthereforeBIOLOGicallyactive.Itisreleasedviathesalivaryglandsandmainlytransformedto5a-dihydrotestosterone.Theconcentrationoftestosteroneinsaliva,reflectshowevertheleveloffreetestosteroneinplasma.PulsatiledynamicsDuetothepulsatiledynamicsofTestosteronesecretionrepeatedsalivasamplingisrecommended.Werecommendcollecting3to5salivasampleswithin2hours.Inthelaboratory,equalvolumesoftheindividualsalivasamplescanbemixed.ThismixedsampleresultsinameanfreeTestosteronevalue,whichrepresentstheactivehormoneconcentrationinareproducibleway.Thereforemeasuringthefreetestosteroneinsalivaisaconvenientnon-invasivemethod.Easiersamplecollectionwithoutrepeatedvenipuncturesandreducedstressonthespecimenofferbenefitstothepatientwhilesensibilityandaccuracyoftheresultsareensured.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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