首页 > IBL > IBL/1,25-dihydroxy Vitamin D ELISA/MG59051/

提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服核对商品价格，订单生效后再付款。

IBL/1,25-dihydroxy Vitamin D ELISA/MG59051/

价格：

登录查看

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号：

MG59051

品牌：

IBL

会员服务：

尊享会员价

贵宾专线

运费优惠

闪电退款

福利优惠

上门换新

友情提示

以上价格仅为参考，请联系客服询价。

免费咨询热线

4000-520-616

产品说明

Kitsize12x8MethodELISAIncubationtime18h,1h,15minStandardrange3-180pg/mLSpecimen/Volumes500µLSerumSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: 1,25-dihydroxyVitaminDELISATheLC-MS/MSisourreferenceThequantificationofthephysiologicallyactiveVitaminDmetabolite1,25-dihydroxyVitaminDisanecessarydiagnostictoolforseveralpathologicalconditionssuchas:KidneydiseasesKidneytransplantationSarcoidosisRicketsOsteomalaciaThequalityofthemeasuringmethod,whichourassayfulfillsahundredpercent,thereforeisindispensable.Thelevelsof1,25-dihydroxyVitaminDinplasmaorserumis100to1000-foldlessthanthatof25-hydroxyVitaminD.Duetoitslowconcentrationsandthepresenceofmanysimilarmetabolites,theexactmeasurementof1,25-dihydroxyvitaminDstillrequirestodoanextractionandseparationstepwithorganicsolventsofthesampleprioranymeasurementsystem.Thisextractionwithorganicsolventsstilltodayisthegoldstandardextractionmethod."Thesuccessiveabandonmentofsampleextraction,chromatography,[…]inimmunoassayshasundoubtedlycontributedtotheinconsistenciesreportedbyFarrelletal,[…]."(GrahamCarter,DEQASorganizer,CarterGD.ClinChem.2012;58(3):486-8.)Theextractionsuppressesthepotentialinterferencefromsamplematrix,heterophilicantibodiesandabnormalconcentrationsofVitaminDbindingproteins.AndtheadditionalseparationonsolidphasecartridgesfurthersuppressestheinterferencefromotherVitaminDmetabolitessuchas25-hydroxyVitaminD,24,25-dihydroxyVitaminD,3-epi-25-hydroxyVitaminDand25,26-dihydroxyVitaminD.ThisleadstogoodcorrelationofourELISAwiththegoldstandardtechnologyLC-MS/MS.CorrelationoftheIBLELISAwiththeLC-MS/MSmethodofQuestDiagnostics(n=11)FurThermoretheELISAwasalsocorrelatedtothestillwidelyusedrADIoimmunoassayfromDiasorin.AlsoheretheELISAshowedagoodfit.CorrelationoftheIBLELISAwiththeradioimmunoassayfromDiasorin(n=118)KeyfeaturesofourELISAare:GoodcorrelationwithLC-MS/MSVerylowLimitofDetection(1.7pg/mL)LimitednumberofincubationstepsDEQASsamplesaccuratelymeasured108%cross-reactivityagainst1,25(OH)2VitaminD2GoldStandardextractionprotocolensuringnointerferenceGeneralinformation1,25(OH)2VitaminDistheactiveformofVitaminDwithregardtotheknownfunctionswhereas25OHVitaminDandVitaminDitselfcanbeexcludedasbeingphysiologicallyfunctional.1,25(OH)2VitaminDstimulatestheintestinalabsorptionofbothcalciumandphosphorus.Italsostimulatesboneresorptionandmineralizationtherebypreventingthedevelopmentofricketsandosteomalacia.1,25(OH)2VitaminDisalsoactiveinothertissuesresponsibleforCalciumtransport(placenta,kidney,mammarygland,...)andendocrineglandssuchasparathyroidglands.1,25(OH)2VitaminDisrapidlymetabolizedanditshalflifeinplasmaisapproximately12h.Itsisfurthermetabolizedtoeithercalcitroicacid,essentiallywithoutanyBIOLOGicalactivity,orvia24-hydroxylationto1,24,25-trihydroxyvitaminD.Thiscompoundhaslessbiologicalactivitythanitsparentandthismetabolicrouteisconsideredasaminorpathway.Thelevelsof1,25(OH)2VitaminDinplasmaorserumis100-to1000-foldlessthanthatof25OHVitaminD.Duetoitslowconcentrationsandthepresenceofmanysimilarmetabolites,themeasurementof1,25(OH)2VitaminDrequiresextractionandseparationbychromatography.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

IBL我们的愿景是通过Tecan技术，产品和支持，为世界各地的每个实验室提供支持。

我们旨在通过无与伦比的专业知识，产品和客户支持来塑造生命科学和临床诊断自动化工作流程的未来。