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IBL/Dehydroepiandrosterone (DHEA) ELISA/RE52221/

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RE52221

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Kitsize12x8MethodELISAIncubationtime1x1h;1x30minStandardrange0.37-30ng/mLSpecimen/Volumes20µLSerumSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: Dehydroepiandrosterone(DHEA)ELISATheLC-MS/MSisourbenchmarkDehydroepiandrosterone(DHEA)anditssulfateesterDHEA-Sarethemostabundantsexsteroidhormonesinhumans,providingalargeprecursorreservoirforthesynthesisofandrogensandestrogens.However,onlythenon-sulfated(free)DHEAshowsBIOLOGicalactivity.IBLInternationaloffersahighlyspecificELISAfordeterminingserumDHEAlevels,whichwascalibratedagainstthereferencemethodLC-MS/MS.Theassay"sdistinguishingfeaturesare:ExceptionalconcordancetoLC-MS/MS:IBLDHEAELISA=0.94xLC-MS/MS+0.13;r²=0.99;N=50VerygoodconcordancetothegoldstandardImmunotechRIA*):IBLDHEAELISA=0.83xImmunotechRIA–0.37;r²=0.96;N=48*)RIAwithextractionExtraction-freeassayAdaptabletoopen-systemELISAanalyzers2kitcontrolsMeasurementofserumDHEAisausefulMarkerofadrenalandrogensynthesis.Decreasedlevelsmayindicateadrenalinsufficiency.Elevatedlevelsarefoundinvariousdiseases,includingadrenalcorticalcarcinoma,tumorswithectopicACTHproduction,adrenalhirsutism,congenitaladrenalhyperplasia,andCushing"sdisease(pituitaryCushing"ssyndrome).Figure1:ExceptionalconcordanceoftheIBLELISAtotheLC-MS/MSreferencemethodMoreover,ourDHEAELISA(RE52221)showsanexcellentagreementwiththegoldstandardassayRIAbyImmunotech.Figure2:ConcordanceoftheIBLELISAtoImmunotechRIA(goldstandard)GeneralInformationEnzymeimmunoassayforthein-vitro-diagnosticquantitativedeterminationofDHEAinhumanserum.Dehydroepiandrosterone(DHEA;androstenolone;3ß-hydroxy-5-androsten-17-one)isaC19steroidproducedintheadrenalcortexand,toalesserextent,gonads.DHEAservesasaprecursorintestosteroneandestrogensynthesis.Duetothepresenceofa17-oxo(ratherthanhydroxyl)group,DHEAhasrelativelyweakandrogenicactivity,whichhasbeenestimatedat~10%thatoftestosterone.Howeverinneonates,peripubertalchildrenandinadultwomen,circulatingDHEAlevelsmaybeseveral-foldhigherthantestosteroneconcentrations,andrapidperipheraltissueconversiontomorepotentandrogens(androstenedioneandtestosterone)andestrogensmayoccur.Moreover,DHEAhasrelativelylowaffinityforsex-hormonebindingglobulin.ThesefactorsmayenhancethephysiologicbiopotencyofDHEA.ThephysiologicroleofDHEAhasnotbeenconclusivelydefined.Avarietyofinvitroeffects,includingantiproliferativeeffectsindifferentcelllinesandeffectsonenzyme-mediatedcellmetabolism,havebeenreported.InvivostudiessuggestthatDHEAmayaffectcholesterolandlipidmetabolism,insulinsensitivityandsecretionandimmunefunction.AbnormalDHEAlevelshavebeenreportedinschizophreniaandobesity.TherapeuticadmiNISTrationofDHEAhasbeenproposedforseveralconditions,includingobesityandcardiovasculardisease.SerumDHEAlevelsarerelativelyhighinthefetusandneonate,lowduringchildhood,andincreaseduringpuberty.IncreasedlevelsofDHEAduringadrenarchemaycontributetothedevelopmentofsecondarysexualhair.SerumDHEAlevelsprogressivelydeclineafterthethirddecadeoflife.NoconsistentchangesinserumDHEAlevelsoccurduringthemenstrualcycleorpregnancy;however,paritymaylowerserumDHEAlevelsinpremenopausalwomen.DHEAhasarapidmetabolicclearancerateascomparedtoitssulfatedconjugate,DHEA-S.Becauseofthis,serumDHEAlevelsare100-1000foldlowerthanDHEA-Slevels.SerumDHEAlevelsincreaseinresponsetoexogenousACTHadministration.MeasurementofserumDHEAisausefulmarkerofadrenalandrogensynthesis.Abnormallylowlevelsmayoccurinhypoadrenalism,andelevatedlevelsoccurinseveralconditions;includingvirilizingadrenaladenomaandcarcinoma,21-hydroxylaseand3ß-hydroxysteroiddehydrogenasedeficienciesandinsomecasesoffemalehirsutism.SinceverylittleDHEAisproducedbythegonads,measurementofDHEAlevelsmayaidinthelocalizationofandrogensourceinvirilizingconditions.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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