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IBL/Free Testosterone ELISA/DB52181/

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DB52181

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4000-520-616

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Kitsize12x8MethodELISAIncubationtime1x1h,1x15minStandardrange0.25-100pg/mLSpecimen/Volumes25µLserumSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: FreeTestosteroneELISAImprovedversionEnzymeimmunoassayforthequantitativedeterminationofFreeTestosteroneinhumanserum.TheimprovedIBLInternationalFreeTestosteroneELISAiscalibratedtotheRIAGoldstandardmethod.BenefitsoftheassayNointerferenceofheterophileantibodiesCalibrationtotheRIAGoldStandardAssayClearlydefinedreferencevaluesAssayadaptedonautomatedplatformsCalibrationtotheGoldStandardTheFreeTestosteroneELISAcorrelatesperfectlytotherADIoimmunoassaygoldstandardmethod.ClearlydefinedreferencevaluesThereferencevaluesmeasuredwiththeIBLInternationalFreeTestosteroneELISAhavebeencarefullyevaluatedinrelationtogenderandage.Serumfrom179apparentlyhealthywomenand179apparentlyhealthymenhasbeencollectedandmeasuredintheFreeTestosteroneELISA.Theexactdataarelistedintheinstructionsforuse,chapter14.Expectedvalues.Itisrecommendedthateachlaboratoryestablishesitsownrangeofnormalvalues.AutomationTheIBLInternationalFreeTestosteroneELISA(DB52181)canbeadaptedondifferentELISAprocessors.OpenautomatedplatformsliketheEVOlyzer®fromTecanhavebeentestedandshowgoodcorrelationtothemanualruns:Thecombineduseofassays,processscriptandautomatehastobevalidatedindividuallyonsitebyeachlaboratory.InformationfromtheInstructionsforUseTestosterone,aC19-Steroid,isthemosteffectivenaturalhormoneinthefamilyofandrogens.Inmales,itismainlyproducedintheLeydigcellsofthetestes,onlyasmallamountisproducedintheadrenalcortex.Onthewhole,adultmaleshave10to20foldhighertestosteroneplasmaconcentrationsthanfemales.Inthecirculation,themainpartoftestosteroneisboundtoplasmaproteinslikesexhormonebindingglobuline(SHBG)andalbumine.Testosteroneisresponsibleforthedevelopmentofsecondarymalesexcharacteristicsanditsmeasurementsarehelpfulinevaluatingthehypogonadalstates.Inwom-en,highlevelsoftestosteronearegenerallyfoundinhirsutismandvirilization,poly-cysticovaries,ovariantumors,adrenaltumorsandadrenalhyperplasia.Inmen,highlevelsoftestosteroneareassociatedtothehypothalamicpituitaryunitdiseases,testiculartumors,congenitaladrenalhyperplasiaandprostatecancer.Lowlevelsoftestosteronecanbefoundinpatientswiththefollowingdiseases:hy-popituitarism,Klinefelter"ssyndrome,testicularfeminization,OrchidectomyandCryptorchidism,enzymaticdefectsandsomeautoimmunediseases.Testosteronecirculatesinthebloodboundtothreeproteins:sexhormonebindingglobulin(60-80%),albuminandcortisolbindingglobulin.Onlyabout1-2%ofthetotalcirculatingtestosteroneremainsunboundorfree.Eventhoughitisstillunderinvestigation,mostresearchersacceptthefreetestosteronedeterminationasameasureoftheBIOLOGicallyactivefraction.Freetestosteronedeterminationsarerecommendedtoovercometheinfluencescausedbyvariationsintransportproteinsonthetotaltestosteroneconcentration.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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