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IBL/25-OH-Vitamin D ELISA/RE53041/

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RE53041

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Kitsize12x8MethodELISAIncubationtime1x90min.,1x30min.,1x15min.Standardrange0-130ng/mLSpecimen/Volumes25µLserumSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: 25-OH-VitaminDELISATheroleofvitaminDindevelopmentofbone-relateddiseasesintheboneandmineralmetabolismhadbeendiscoveredsincemanyyears.ClinicalrelevanceTheaccurateassessmentofaperson’svitaminDstatusreliesonthemeasurementof25-OH-vitaminDtotal(25-OH-vitaminD2+25-OH-vitaminD3)levels.ThereforeourassayhasbeendevelopedtodetectbothD2andD3variants.TheroleofvitaminDinregulatingthecalcium-phosphorusbalancefornormalbonemineralizationiswellknownsincemanyyears.Thusthedeterminationof25-OH-vitaminDhaslongbeenusedfordiagnosisandmonitoringtherapyofrickets,renalosteodystrophy,osteomalaciaandpostmenopausalosteoporosis.Morerecentstudiesindicatethatinsufficient(seetablebelow)vitaminDlevelsarecorrelatedwithincreasedriskofdevelopingotherdiseases,suchascancer,hypertension,diabetes,cardiovasculardiseasesandmanymore.SuggestedreferencevaluesDeficiency<10ng/mLInsufficiency10-30ng/mLSufficiency30-100ng/mLToxicity>100ng/mLCorrelationtoLC-MS/MSTheIBL-International25-OH-VitaminDELISAiscorrelatedagainsttheLC-MS/MSreferencemethod.Thecorrelationwasperformedon135serumsamplescomparingtheIBL-International25-OH-VitaminDELISAtotheLC-MS/MS.Theregressionanalysisshowedaslopeof1.042,aninterceptof-3.731ng/mLwithacorrelationofR=0.92.Graph1:CorrelationtoLC-MS/MSAutomationTheIBLInternational25-OH-VitaminDELISAhasbeendevelopedtobeadaptabletoopenELISAprocessors.Anin-housecorrelationhasbeenperformedcomparingthemanualwork-upofthe25-OH-VitaminDELISAandtheautomatedwork-upofthe25-OH-VitaminDELISAontheTECANFreedomEVOlyzer®.Theregressionanalysisshowedaslopeof0.9737,aninterceptof-0.269ng/mLwithacorrelationofR=0.99.Graph2:CorrelationofautomatedEVOlyzer®testingtomanualwork-upAdvantagesofourELISAExcellentcorrelationtotheLC-MS/MSreferencemethodRE53041=1.042xLCMS-3.731,R=0.92DirectassaywithoutextractionReady-to-usereagentsEasilyadaptableonopenELISAplatformsForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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