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IBL/Dentin Matrix Protein 1 (rat) ELISA/JP27363/

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JP27363

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Kitsize12x8MethodELISAIncubationtime1x1h,2x30min.Standardrange39.1-2500pg/mLSpecimen/Volumes5µLserum,plasmaSubstrate/isotopeTMB450nmRegulatoryStatus:ForresearchuseonlyDetailsfor: DentinMatrixProtein1(rat)ELISADentinMatrixProtein1(DMP1)isaconstitutiveproteinintheextracellularmatrixofosteocytesanditbelongstotheSmallIntegrin-BindingLigand,N-linkedGlycoprotein(SIBLING)family.Itissynthesizedasafull-length105kDaproproteinandisdividedintoa37kDaNterminalproteinfragmentanda57kDaCterminalproteinfragmentduringthesecretionprocess.Ithasalotofacidicdomainsanditbindswithcalciumasitbecomesnegativelychargedwithintissuesandithasanimportantroleforbonemineralization.DMP1expressesinosteocyteswhileotherconstitutiveproteinsintheextracellularmatrixsuchasOsteopontin,OsteocalcinandBonesialoproteinexpressinosteoblastcells.Osteocyteswhicharethemainconstituentcellsofboneregulateactivitiesofosteoclasticcellsandosteoblastcellsanditadjuststheboneremodelingprocess.Theyalsoactasendocrinecells,whichsecreteFGF-23.DMP1inbloodisconsideredasacandidatebioMarkerreflectingosteocyteactivityanditisalsosuggestedtoaddusefulinformationforresearchofbonemetabolismandphosphatemetabolism.TheconcentrationofDMP1inratbloodcanbequantitativelymeasuredbythisELISAkit.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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