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IBL/GLP-1 (active forms) ELISA/JP27784/

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JP27784

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Kitsize12x8MethodELISAIncubationtime2x1h,1x30minStandardrange1.25-80pmol/LSpecimen/Volumes100µLEDTAplasmaSubstrate/isotopeTMB450nmRegulatoryStatus:ForresearchuseonlyDetailsfor: GLP-1(activeforms)ELISATheguthormoneglucagon-likepeptide-1(GLP-1)issecretedinresponsetonutrientintakeandplaysanessentialroleintheregulationofglucosehomeostasis.TherearemultipleformsofGLP-1,amongwhichGLP-1(7-37)andGLP-1(7-36)NH2areconsideredtobeBIOLOGicallyactive.AccuratemeasurementofbothformsofactiveGLP-1isnotonlyimportantforunderstandingthephysiologicalmechanismsofbloodglucoseregulationinhealthypeopleandtype2diabetespatients,butitisalsoofutmostimportanceforbothclinicalandpreclinicalstudiesofGLP-1agoNISTsandDPP4inhibitors(e.g.Sitagliptin®,Saxagliptin®).AmongthecurrentlyavailablemethodsformeasuringactiveGLP-1peptides,immunoassaysaregreatlyfavoredbecauseoftheirsensitivity.However,mostimmunoassaysstillrequireanextractionstepofactiveGLP-1whichistediousandtime-consuming.Tomeettheseneeds,areliableanduser-friendlyassaywithahighsensitivityisessentialduetolowplasmalevelsoftheactiveformGLP-1.AdvantagesofourassaySensitivity:0.10(pmol/L)Specificity:GLP-1(7-36)NH2:100%GLP-1(7-37):100%Others< 0.32%Assaytime:2.5hoursNoextractionneededGLP-1activeformsELISAwithoutextractionIBLInternationalishappytopresenttheGLP-1activeformsELISAwithoutextractionforthereliablemeasurementofbiologicallyactiveformsofGLP-1.OurassayneedsnoextractionstepanddetectsbothformsoftheactiveGLP-1withhighsensitivity.*distributedbyIBLInternationalForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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