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IBL/IA2-GAD65 Screen ELISA/RE70391/

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RE70391

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Kitsize12x8MethodELISAIncubationtime1x18h,2x30min.Standardrange0-500IU/ml;cut-off30IU/mlSpecimen/Volumes75µlserumSubstrate/isotopeTMB450nmRegulatoryStatus:ForresearchuseonlyDetailsfor: IA2-GAD65ScreenELISATheIBLIA2-GAD65ScreenELISAisasolidphaseenzymeimmunoassayemployingafusionproteinofrecombinanthumantyrosinephosphatase-relatedproteinisletantigen2(IA2)andrecombinanthumanglutamatedecarboxylase65kDaisoform(GAD65)forthequantitativeandqualitativedetectionofantibodiesagainstisletcellsinhumanserum.Theassayisatoolinthediagnosisoftype1diabetesmellitus.Theinsulindependentdiabetesmellitus,alsocalledtypeIdiabetes(T1DM),isachronicautoimmunediseaseresultingfromtheautoimmunedestructionofinsulinproducingß-cellsofLangerhans`islets.Itischaracterizedbylargelyorcompletelackofinsulinproductionandthepresenceofß-cellautoantibodies.Theantigensrecognizedbytheseantibodiesincludeinsulin,glutamatedecarboxylase(GAD65kDaisoform)andtyrosinephosphatase-relatedproteinisletantigen2(IA2).AutoantibodiesagainstisletcellantigensareimportantpreclinicalMarkersastheymaybepresentforyearsbeforediagnosisofdiabetesandatatimewhereothermetabolicassaysshownormaldiagnosticfindings.RecentstudiesshowthatacombinedtestingforantibodiesagainstGAD65andIA2detectsmorethan90%ofnewonsettype1diabetespatients.TheIBLELISAIA2-GAD65ScreenallowsthesimultaneousdetectionofautoantibodiesagainstGAD65andIA-2.It’sanidealscreeningassaytoidentifyindividualsofhighriskfortype1diabetesmellitus.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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