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IBL/Acylated Ghrelin (human) 384 well ELISA/CMA05106-384/

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CMA05106-384

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4000-520-616

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Kitsize384determinationsMethodELISAIncubationtime1x3hatRT;1xatRTStandardrange2-250pg/mLSpecimen/Volumes25µLSubstrate/isotopeER405-414nmRegulatoryStatus:ForresearchuseonlyDetailsfor: AcylatedGhrelin(human)384wellELISAThisassayhasbeendevelopedtoscreenGOATactivitybymonitoringAcylatedGhrelinproductionincellculturemedia.Ghrelindiscoveredin1999,isfastbecominganendocrinologytargetofthemillennium.Ghrelin,identifiedinratstomachasanendogenousligandfortheGHsecretagoguereceptor,ismainlyproducedinstomach,buthasbeendemonstratedinmanyotherorgans.InadditiontoGH-releasingpropertiesanditsorexantaction,Ghrelincouldactasanhormonehavingeffectsongastricmotility(similaritywiththepeptidehormonemotilin),acidicsecretion,cardiovascularaction,antiproliferativeeffects,pancreaticandglucosemetabolismfunction,sleep...GhrelingeneraisestomRNAprepro-ghrelinof117aminoacids.ThisprecursorisprocessedintoGhrelin,28aminoacids(human).Beforebeingsecreted,thispeptideisoctanoylatedatSer3byGOAT(GhrelinOctanoylAcylTransferase).ThisstepisessentialforBIOLOGicalactivitymakingGOATaperfecttargetfordrugsinfeedingbehaviour.Interestingly,thepotentialtherapeuticimportanceofthishormoneisnotrestrictedtoregulationoffoodintakebutalsoincachexia(relatedtocancertreatment,anorexianervosaorischemia)gastricmotilityandmaybeinvolvedinosteoporosis,somatopause,infertilityandovulationinduction,neurologicaldisorders(Alcoholism,PostTraumaticStressdisorders...)andcardiovasculardiseases.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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