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IBL/MetCombi Plasma ELISA (Metanephrine / Normetanephrine)/RE59202/

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RE59202

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4000-520-616

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Kitsize2x(12x8)MethodELISAIncubationtime1x1h,1x2h,2x30minStandardrangeMN:0-2500pg/mL;NMN:0-3000pg/mLSpecimen/Volumes200µlEDTAPlasmaSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: MetCombiPlasmaELISA(Metanephrine/Normetanephrine)FastandreliableassayforassessingplasmafreemetanephrinesEnzymeimmunoassayforthequantitativedeterminationoffreemetanephrineandnormetanephrineinhumanplasma.Catecholaminesandtheirmetabolites,metanephrineandnormetanephrine,aresecretedinexcessiveamountbyvarioustumors,includingparaganglioma,neuroblastoma,andinparticularpheochromocytoma.TheclinicalmanifestationofthesetumorsishighlyvariableandoftenresemblessymptomsassociatedwithcommonmalADIes,predominantlyhypertension,headacheandsweating.Biochemicaltestingforpheochromocytomaisindicatednotonlyinsymptomaticpatients,butalsoinpatientswithadrenalincidentalomasoridentifiedgeneticpredispositions.Itinvolvesmeasurementsofcatecholamines(adrenaline,noradrenalineanddopamine)andtheirmetabolites(metanephrine,normetanephrineandvanillylmandelicacid)inplasmaorurine.NumerousstudieshaveshownthatfreemetanephrinesinplasmaarethebestMarkersforthediagnosisofpheochromocytomes(Grossmannetal,2006;Lendersetal.,2005;Eisenhoferetal.,2000) SensitivitySpecificityPlasma–freemetanephrines99%89%Urinary–fractionatedmetanephrines97%69%Urinarycatecholamines86%88%Plasmacatecholamines84%83%Totalurinarymetanephrines77%93%Vanillylmandelicacid(VMA)64%95%Table1Lendersetal.Lancet(2005)IBLInternational’sMetCombiPlasmaELISAhasbeenimprovedShorterassaytime:ca.4.5hCalibratedtoLC–MS/MSAutomatedtestprocedure(DSX)Smallersamplevolume:200µLHighersensitivity: Metanephrine:7pg⁄mL Normetanephrine:7pg/mL

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