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IBL/hCG ELISA/RE52191/

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RE52191

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4000-520-616

产品说明

Kitsize12x8MethodELISAIncubationtime1x30min,1x10minStandardrange5-1000mIU/mLSpecimen/Volumes25µLSerum,plasmaSubstrate/isotopeTMB,450nmRegulatoryStatus:EU:CEDetailsfor: hCGELISAThehCGELISAisanenzymeimmunoassayforthequantitativeinvitrodiagnosticmeasurementofintacthumanchorionicgonadotropin(hCG)inserumorplasma.ChorionicGonadotropin(hCG)isaglycoproteinhormonewhichisnormallyproducedbytheplacentaduringpregnancy.Afterconception,thehCGconcentrationincreasesrapidlytoreachapeakneartheendofthefirsttrimester.Highconcentrationsareobservedthroughoutpregnancy.Afterdelivery,hCGlevelsfallrapidlyandbecomeundetectableafterafewdays.StructurallyintacthCGmoleculesarecomposedofanalphaandabetasubunitwithamolucularweightof38.4kDa.Thealphasubunitisnearlyidenticaltothealphasubunitsofotherglycoproteinhormones,suchasThyroidStimulatingHormone(TSH),LuteinizingHormone(LH),andFollicleStimulatingHormone(FSH):ThedifferencesinthebetasubunitoftherespectivehormonesaccountfortheirBIOLOGicalspecificityandimmunochemicaldistinctiveness.MonoclonalantibodiesrecognizinguniquesitesonthebetachainofthehCGmoleculeareessentialfordifferentiationbetweenhCGandLH,FSHandTSH.hCGAssaysareusedfortheearlydetectionofpregnancy.1.InadditiontotheelevatedhCGlevelsduringpregnancy,highconcentrationsofhCGmaybeassociatedwithneoplasmsoftrophoblasticandnontrophoblasticoriginsuchashydatiformmole,chorionepithelioma,embryonalcellcarcinoma,andmanyothers.2.HCGiscommonlyelevatedindifferenttesticulartumorsandisthususedasatumorMarkerfortesticulartumorsincombinationwithAFP.ThereisagoodcorrelationbetweenchangesinhCGlevelsandresponsetotherapy.3.Extragonadalgerm-cellcancersintheabsenceofclinicallyorultrasonographicallydetectabletesticularabnormalitieshavebeeinobservedaswell.Over50%ofpatientswithmalignantinsulinomashaveelevatedhCGlevels:thehormoneisnotdetectedinassociationwithbenignadenomas.EctopicsecretionofhCGalsohavebeenfoundinasmallpercentageofpatientswithadenocarcinomaoftheovary,pancreasandstomach,hepatomas,andislet-cellcarcinomas.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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