首页 > IBL > IBL/IRT neonatal Luminescence Immunoassay (2400 det.)/RE68019/

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IBL/IRT neonatal Luminescence Immunoassay (2400 det.)/RE68019/

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RE68019

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4000-520-616

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Kitsize480determinationsMethodLuminescenceImmunoassayIncubationtime1x2hStandardrange0-459ng/mlSpecimen/VolumesbloodspotSubstrate/isotopeChemiluminescenceReagentAPRegulatoryStatus:EU:CEDetailsfor: IRTneonatalLuminescenceImmunoassay(2400det.)LuminescenceImmunoassayforthein-vitro-diagnosticquantitativedeterminationofimmunoreactivetrypsin(IRT)inhumannewbornbloodspotsamples.Forneonatalscreeningofcysticfibrosis(CF).Cysticfibrosis(CF)isoneofthemostcommonautosomalrecessivediseasescausedbymutationsintheCFtransmembraneconductanceregulatorgene(CFTR).CFcanresultindeathatanearlyage,primarilyfromprogressivelungdisease,althoughanumberoforgansareofteninvolved.CFoccursatanincidenceofapproximately1:2000-1:5000livebirthsinEuropeandNorthAmerica.ThefirstEuropeanexperiencesincysticfibrosis(CF)newbornscreening(NBS)datebacktotheearlynineteenseventies,withpioneeringprogrammesexaminingthealbumincontentofmeconium.TheelevationofImmunoreactiveTrypsin(IRT)inthebloodofneonateswithCFanditsmeasurementindriedbloodspotswasfirstdescribedin1979.Duringthefollowingdecade,thedeterminationofIRTlevelsinheelbloodwasintroducedinseveralcountries.FurtherimprovementwaspossIBLeaftercloningoftheCFTRgenein1989andsubsequentidentificationofcommonpopulationspecificCFTRgenemutationsallowedinclusionofDNAtestingintoscreeningprotocols.StudieshaveshownthatearlydiagnosisofCFthroughneonatalscreening,combinedwithaggressivenutritionaltherapy,cansignificantlyenhancelong-termnutritionalstatus.ThetwomostcommonprotocolsforCFscreeningare(a)measuringIRTononesampleandthenonasecondsample,and(b)IRTtestingfollowedbyDNAmutationanalysisonthesamesample.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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