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IBL/Progesterone Saliva ELISA/RE52281/

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RE52281

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Kitsize12x8TestsMethodELISAIncubationtime1x1h,1x30minStandardrange25-5000pg/mLSpecimen/Volumes50µLSalivaSubstrate/isotopeTMB,450nmRegulatoryStatus:EU:CEDetailsfor: ProgesteroneSalivaELISAThenewIBLInternationalProgesteroneSalivaELISAiscalibratedtotheLC-MS/MSreferencemethodandtotheluminescenceImmunoassay(IBLProgesteroneSalivaLUM,RE62021).Thisnewassayrequiresaslowas50µLSalivasamplevolumeandhasabroadstandardrangeupto5,000pg/mL.BenefitofStandardizedreagentsOurSalivaELISAproductlinehasbeenrevisedsothatthemainsteroids(cortisol,testosterone,progesterone,DHEA)maybeprocessedinasimilarway:Thesubstrate,washingandstopsolutionsareinterchangeable;Theenzymeconjugate,thesubstrateandstopsolutionsareuniformlyPipettedwith100µL;Thekitscontainasfewas6standardsand2controls;Only50µLsamplevolumeisrequired;Allkitsusethesamesamplediluentbuffer.Thankstothesimilarprocessingandassembling,theassayscaneasilybeper-formedincombination.ApplicationfieldsThetwomainindicationsforprogesteronemeasurementsaretheCorpusLuteuminsufficiencyaswellastheanovulatoricalmenstrualcyclesinfemales.Inanti-agingmedicineitmightbenecessarytoevaluatetheProgesteronelevelbeforeandduringthereplacementtherapy.PulsatiledynamicsDuetothepulsatiledynamicsofsteroidsecretionrepeatedsalivasamplingisrec-ommended.IBLrecommendscollecting3to5salivasampleswithin2hours.Inthelaboratory,equalvolumesoftheindividualsalivasamplescanbemixed.ThismixedsampleresultsinameanfreeProgesteronevalue,whichrepresentstheactivehormoneconcentrationinareproducibleway.InformationfromtheInstructionsforUseMeasurementsobtainedbythisassaymaybeusedinthediagnosisandtreatmentofdisordersoftheovariesorplacentaandcanbeusedasanaidforpredictionofovulation.TheProgesteronelevelinsalivarepresentstheconcentrationoftheactivefreeProgesterone.Progesterone(4-pregnene-3,20-dione)isaC21steroidhormonecontainingaketo-group(atC-3)andadoublebondbetweenC-4andC-5.Likeothersteroids,itissynthesizedfromcholesterolviaaseriesofenzyme-mediatedsteps.ThesteroidhormoneProgesteroneisafemalesexhormonewhich,inconjunctionwithestro-gens,regulatestheaccessoryorgansduringthemenstrualcycleanditisparticu-larlyimportantinpreparingtheendometriumfortheimplantationoftheblastocyteandinmaintainingpregnancy.Innon-pregnantwomenprogesteroneismainlysecretedbythecorpusluteumwhereasinpregnancytheplacentabecomesthemajorsource.Minorsourcesforprogesteronearetheadrenalcortexforbothsexesandthetestesformales.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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