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IBL/17ß-Estradiol Saliva ELISA/30121045/
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Kitsize12x8MethodELISAIncubationtime2x1h,1x30minStandardrange2-64pg/mLSpecimen/Volumes50µLSalivaSubstrate/isotopeTMB450nmRegulatoryStatus:IVDDetailsfor: 17ß-EstradiolSalivaELISAEnzymeimmunoassayforthequantitativemeasurementof17beta-Estradiolinhumansaliva.Resultsobtainedbythisdevicemaybeusedinthediagnosisandtreatmentofvarioushormonalsexualdisorders.Thistestisnotintendedforassessingplacentalfunctionincomplicatedpregnancy.17β-Estradiol[1,3,5(10)-estratriene-3,17β-diol;E2]isaC18steroidhormoneandthemostimportantnaturalestrogen.Itispresentinfemalesandmales.Intheformer,theestrogensstimulatethegrowthofsexorgansandthedevelopmentofsecondarysexualcharacteristics,andtheyalsoaffectthegonadotropinsecretion.Inthemales,theroleof17β-Estradiolislesswelldefinedalthoughitseemstobeinvolvedintheregulationofgonadotropinsecretion.Innon-pregnantwomen17β-Estradiolisalmostexclusivelyproducedbyovary.Especiallyafterhavingchangedintothemenopause,estrogenesareproducedbytheliver,thebrain,themusclesandbytheadiposetissue,too.Moreover,themeasurementof17β-Estradiolishelpfultodeterminealackofestrogenes,whichmaybeexpressedasdelayedpuberty,primaryandsecondaryamenorrhoea,andoccursinthemenopause.Otherhormoneassaysarerequiredforproperinterpretationanddifferentialdiagnosis.Theconcentrationofgonadotropinshouldbemeasuredtofindtheoriginofthelackofestrogenes(synthesisorregulation).Thedeterminationof17β-Estradiolisusefulforthediagnosisofpubertaspraecoxofgirls.Concerningmen,thelevelof17β-Estradiolmaybeusedforthedifferentialdiagnosisofgynecomastia.Beingprincipallyboundtosexhormonebindingglobuline(SHBG)andtoserumalbumin,only1-3%ofestradiolcirculatinginplasmaispresentinitsfreeform.Onlythisportionrepresentstheactivepartintheendocrineregulation.Thefreehormoneisreleasedinequalamountsinsaliva.Becausethefluctuationsofthe17β-Estradiollevelsdependsalsoonindividualsituations,itisveryconvenienttogetahormoneprofilebyrepeatedlycollectingsalivasamples.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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