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IBL/Interleukin-2 (IL-2) High Sensitivity ELISA/BE58021/

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Kitsize12x8MethodELISAIncubationtime1x2h,1x1h,1x15min,1x30min,1x15minStandardrange0.94-60pg/mlSpecimen/Volumes50µlserum,plasma,supernatants,saliva,etcSubstrate/isotopeTMB450nmRegulatoryStatus:ForresearchuseonlyDetailsfor: Interleukin-2(IL-2)HighSensitivityELISAThisHighSensitivityELISAenablestheanalysisofInterleukin-4inlowconcentrationsfromserum,plasma,orcellculturesamples.TheHighSensitivityELISAKitsofferaccuratequantificationbelowconventionalELISAlimits.ThisismadepossIBLebytheirinherentdesignbyamplifyingthesignal.Interleukin-2(IL-2)playsacentralroleintheactivationandproliferationoflymphocytesthathavebeenprimedbyantigens.IL-2playsapivotalroleinfortheexpansionofmostT-cells,naturalkillercellsandBcellsduringcertainphasesoftheirresponse.IL-2isa15kDaglycoproteinencodedbyasinglegenelocatedintheq26-28regionofhumanchromosome4.IL-2geneexpressionisregulatedatthetranscriptionallevelbyseveralactivationpathways.Antigen-specificproliferationofhelperandcytotoxicT-lymphocytesfollowingstimulationiscriticallydependentonIL-2expression,secretion,andbindingtoreceptorsforIL-2inducedinanautocrinefashiononthesurfaceofT-cells.Apartfromitsmostimportantroletomediateantigen-specificT-lymphocyteproliferation,IL-2modulatestheexpressionofinterferon-gammaandmajorhistocompatibilityantigens,stimulatesproliferationanddifferentiationofactivatedB-cells,augmentsnaturalkillercellactivityandinhibitsgranulocyte-macrophagecolonyformation.AlterationsintheABIlityofT-cellstosynthesizeIL-2havebeenobservedinphysiologicandpathologicstates.BecauseofthecentralroleofIL-2inimmuneresponse,IL-2turnedouttobeaveryimportantmoleculefordiagnosticandtherapeuticimplications.IL-2displaysantitumoraleffects,thusbeingusedincancertherapy.MonitoringofIL-2levelsinserumprovidesmoredetailedinsightsinseveralpathologicalsituationssuchascancer,infectiousdiseases,transplantrejection,multiplesclerosis,rheumatoidarthritis,systemiclupuserythematosusandtypeIdiabetes.Synonyms:T-cellgrowthfactor,TCGF,AldesleukinForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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