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IBL/Interleukin-6 (IL-6) ELISA/BE53061/

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BE53061

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4000-520-616

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Kitsize12x8MethodELISAIncubationtime1x2h,1x1h,1x10minStandardrange1.56-100pg/mLSpecimen/Volumes50µLserum,plasma,cellculturesupernatantSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: Interleukin-6(IL-6)ELISAInterleukin-6(IL-6)isamulti-functionalcytokinethatregulatesimmuneresponses,acutephasereactionsandhematopoiesisandmayplayacentralroleinhostdefensemechanisms.IL-6isusuallynotproducedconstitutivelybynormalcells,butitsexpressionisreADIlyinducedbyavarietyofcytokines,lipopolysaccharideorviralinfections.IL-6isapleiotropiccytokineproducedbyavarietyofcells.Itactsonawiderangeoftissues,exertinggrowth-induction,growth-inhibition,anddifferentiationrespectively,dependingonthenatureofthetargetcells.TheabnormalproductionofIL-6wasfirstsuggestedtoberelatedtopolyclonalB-cellactivationwithautoantibodyproductioninpatientswithcardiacmyxoma.Sincethen,IL-6hasbeensuggestedtobeinvolvedinthepathogenesisofavarietyofdiseases.MeasurementofIL-6levelsinserumandotherbodyfluidsthusprovidesmoredetailedinsightsintovariouspathologicalsituations.InfectionsBodyfluidsofpatientswithacutelocalbacterialorviralinfectionsandserumofpatientswithgram-negativeorpositivebacteremiacontainelevatedlevelsofBIOLOGicallyactiveIL-6.ObstetricInfectionsIL-6hasemergedasareportercytokineforintraamnioticinfection.Diseasesassociatedwithanalteredimmunesystem(polyclonalB-cellabnormalitiesorautoimmunediseases)ElevatedlevelsofcirculatingIL-6havebeendetectedinpatientswithcardiacmyxoma,Castleman"sdisease,rheumatoidarthritis,IgMgammopathyandinthosewithacquiredimmunodeficiencysyndromeaswellasalcoholiclivercirrhosis.ProliferativediseasesElevatedplasmalevelsofIL-6areobservedinpatientswithpsoriasisandmesangialproliferativeglomerulonephritis.NeoplasticDiseasesIncreasedsystemiclevelsofIL-6havebeendetectedinpatientswithmultiplemyeloma,otherB-celldyscrasias,Lennert"sTlymphoma,Castleman"sdisease,renalcellcarcinomaandvariousothersolidtumors.InflammatoryresponsesIL-6isinvolvedintheinductionofacutephaseproteinsandinductionoffever.ElevatedserumlevelsofIL-6arealsofoundinpatientswithsevereburns,inserumandplasmaasaMarkerforpredictingpostoperativecomplications,inserumandurineofrecipientsofkidneytransplantsbeforerejection,intheserumofsepticshockpatientsandinpatientswithinflammatoryarthritisandtraumaticarthritis.Synonyms:B-cellstimulatoryfactor-2,BSF-2,Interferonbeta-2,Hybridomagrowthfactor,CTLdifferentiationfactor,CDFForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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