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IBL/17ß-Estradiol Saliva ELISA/RE52601/

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RE52601

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4000-520-616

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Kitsize12x8MethodELISAIncubationtime1x30min,1x2h,1x30min.Standardrange1-100pg/mLSpecimen/Volumes100µLsalivaSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: 17ß-EstradiolSalivaELISAELISAfortheinvitrodiagnosticquantitativemeasurementof17beta-estradiolinhumansaliva.Measurementsobtainedbythisdevicemaybeusedinthediagnosisandtreatmentofvarioushormonalsexualdisordersandcanbeusedtoevaluateovarianfunction.Thistestisnotintendedforassessingplacentalfunctionincomplicatedpregnancy.17b-Estradiol[1,3,5(10)-estratriene-3,17b-diol;E2]isaC18steroidhormoneandthemostimportantnaturalestrogen.Itispresentinfemalesandmales.Intheformer,theestrogensstimulatethegrowthofsexorgansandthedevelopmentofsecondarysexualcharacteristics,andtheyalsoaffectthegonadotropinsecretion.Inthemales,theroleof17b-Estradiolislesswelldefinedalthoughitseemstobeinvolvedintheregulationofgonadotropinsecretion.Innon-pregnantwomen17b-Estradiolisalmostexclusivelyproducedbyovary.Especiallyafterhavingchangedintothemenopause,estrogenesareproducedbytheliver,thebrain,themusclesandbytheadiposetissue,too.Theserialmeasurementof17b-Estradiolduringtheovulatorycycleishelpfultoevaluatetheovarianfunctionforaccurateassessmentoffolliculargrowthandalsoformonitoringtheexponentialincreasein17b-Estradioloccuringeitherinspontaneouscyclesorcyclesstimulatedforinductionofovulationor„superovulation“before„invitro“fertilization.Moreover,themeasurementof17b-Estradiolishelpfultodeterminealackofestrogenes,whichmaybeexpressedasdelayedpuberty,primaryandsecondaryamenorrhoea,andoccursinthemenopause.Otherhormoneassaysarerequiredforproperinterpretationanddifferentialdiagnosis.Theconcentrationofgonadotropinshouldbemeasuredtofindtheoriginofthelackofestrogenes(synthesisorregulation).Thedeterminationof17b-Estradiolisusefulforthediagnosisofpubertaspraecoxofgirls.Concerningmen,thelevelof17b-Estradiolmaybeusedforthedifferentialdiagnosisofgynecomastia.Beingprincipallyboundtosexhormonebindingglobuline(SHBG)andtoserumalbumin,only1-3%ofestradiolcirculatinginplasmaispresentinitsfreeform.Onlythisportionrepresentstheactivepartintheendocrineregulation.Thefreehormoneisreleasedinequalamountsinsaliva.Becausethefluctuationsofthe17b-Estradiollevelsdependsalsoonindividualsituations,itisveryconvenienttogetahormoneprofilebyrepeatedlycollectingsalivasamples.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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