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IBL/1-Methylhistamine ELISA/RE59231/

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Kitsize12x8MethodELISAIncubationtime1x60min,1x30min,1x20min,1x15-20minStandardrange10-1000ng/mLSpecimen/VolumesUrineSubstrate/isotopeTMB450nmRegulatoryStatus:EU:CEDetailsfor: 1-MethylhistamineELISATheonlycommerciallyavailableELISAassayIBLInternationalprovidesareliableandfastimmunoassayforthedeterminationofthemainmetaboliteofhistamine,1-methylhistamine(N-methylhistamine),invariousBIOLOGicalfluids.Extensiveresearchhasshownthathistamine[2-(4-imidazolyl)-ethylamine]istheprinciplemediatorofmultiplebiologicalprocessesincludingneuromodulation,inflammation,gastricacidsecretionandtheregulationofimmunefunction.Itismainlystoredinthemetachromaticgranulaofmastcellsandthebasophilicleukocytes,andreleaseduponstimulation.HistamineispredominantlymetabolizedviaN-methytransfereseto1-methylhistamine,whichisfurtherprocessedtoN-methylimidazoleaceticacid,whiletheremainderismetabolizedbydiamineoxidasetoimidazoleaceticacid(Fig.1).Theurinary1-methylhistaminelevelcorrelateswellwiththehistaminelevelsinplasmaandurine(1,2),andismorestablethanhistamineinplasma.Thedeterminationofurinary1-methylhistaminefindsitapplicationprimarilyinthediagnosisandmonitoringofmast-celldisorders,includingmastocytosis,anaphylaxis,andotherseveresystemicallergicreactions.UniquefeaturesTheonlycommerciallyavailableELISASensitivity:urine:2.5ng/mL,plasmaandcellculture:0.13ng/mLCalibratedtoLC-MS/MS(Fig.2)Totalassaytimeisonly2hoursAlsoapplicableforplasmaandcellculturesamplesFigure1:Histaminemetabolism.Synthesisofhistamineviadecarboxylationofhistidine(1).Metabolismofhistamineeitherbydiamineoxidase(DAO)(2)orbyhistamine-N-methyltransferase(HNMT)(3).Negativefeedbackinhibitionofbothenzymesbytheirrespectiveproducts(4).FurtherprocessingofN-MethylhistaminebymonoamineoxidaseB(MAOB)(5)orbyDAO(6).3)Figure2:MethodcomparisonbetweentheIBLInternational1-methylhistamineELISAandLC-MS/MSshowsanexcellentcorrelation:IBLInternationalinternaldata.)Literature1.HermannK.etal.Measurementandcharacterizationofhistamineandmethylhistamineinhumanurineunderhistamine-richandhistamine-poordiets.IntArchAllergyImmunol.1993;101(1):13-9.2.BelicA.etal.Interdependenceofhistamineandmethylhistaminekinetics:modellingandsimulationapproach.ComputBiolMed.1999Nov;29(6):361-75.3.MaintzL.etal.Histamineandhistamineintolerance.AmJClinNutr2007;85:1185–96.DistributedbyIBLInternational.ForconcretedatapleaseconsulttheInstructionforUseinthedownloadboxontherightside.

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